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What's the best competent cell packaging format for you?    Whether you have to perform
one transformation,
96 transformations,
or something in between,
we have the packaging to meet your needs. | | Singles™ Competent Cells | Singles are designed for the ultimate in convenience and reliability in plasmid transformation. The cells are provided in single-use 50-µl aliquots. The Singles format eliminates the need to dispense, freeze/thaw or waste partially used vials, thus saving time and increasing performance. For use, simply thaw, add DNA, heat shock for 30 seconds, place on ice for 2 minutes and plate. | | Standard Competent Cells | Standard competent cells are available in 0.2-ml aliquots, with 2 or 5 tubes per kit. More than 40 competent strains are available, including the popular NovaBlue strain for general-purpose cloning, and the widest selection of protein expression strains available in the industry. | | Competent Cell Sets | Host strains are available in sets for convenient, efficient optimization of protein expression. Each set contains two 0.2-ml aliquots of each included strain. Standard sets include a selection of T7 expression strains (lDE3 lysogens) and non-lDE3 lysogens.
See also Special Packaging Configurations | | | | Quarters™ Competent Cells | These novel formats enable convenient, efficient transformation of T7-based expression plasmids for high-throughput optimization. Cells are dispensed in single-use 20-µl aliquots into 24-well quarter sections of a 96-well polypropylene plate. Up to four Quarters can be placed into the HT96™ Isothermal Block to create an array of 1, 2, 3 or 4 different competent cell strains. | | QuarterPack™ Competent Cell Arrays | QuarterPack Competent Cell Arrays come as sets of 4 different Quarters. QuarterPack Competent Cell Array 1 is designed to analyze whether rare codons or target protein toxicity are affection protein yield. Array 2 provides an analysis of a broad range of pET expression hosts. BL21 establishes the baseline for yield in a protease-deficient host, Rosetta™ alleviates codon bias if present, Origami™ is compatible with disulfide bond formation in the target protein and Rosetta-gami™ combines all of the previous attributes. Array 3 represents the stringent (pLysS) version of Array 2 and is designed to decrease background expression of target proteins that may be toxic to E. coli.
See also Special Packaging Configurations | | HT96™ Competent Cells | Designed for HT cloning and protein expression, HT96 competent cells are dispensed in single-use 20-µl aliquots into a 96-well polypropylene plate. Groups of 24 wells can be detached to process fewer samples. Wells are individually sealed and have raised rims to prevent cross-contamination. Seals can be easily peeled off before use. | | |
| | | Singles
No. of rxns | Standard
Volume (ml) | | | HT96
No. of 96-well Plates | | | | | | | | Set | 24 | | | 20 | | Competent Cells | 11 ×
50 µl | 22 ×
50 µl | 2 ×
0.2 ml | 5 ×
0.2 ml | 3 ×
(2 ×
0.2 ml) | 24 ×
20 µl | 96 ×
20 µl | 4 ×
(96 ×
20 µl) | 20 ×
(96 ×
20 µl) | | Test Plasmid | 10 µl | 10 µl | 10 µl | 10 µl | 10 µl | 10 µl | 10 µl | 2 ×
10 µl | 10 ×
10 µl | | SOC Medium | 2 ×
2 ml | 4 ×
2 ml | 2 ×
2 ml | 4 ×
2 ml | 4 ×
2 ml | 2 ×
2 ml | 14 ml | 4 ×
14 ml | 20 ×
14 ml | | 8 Cap Strip | | | | | | pkg/12 | pkg/12 | 4 ×
pkg/12 | 20 ×
pkg/12 | | Reagent Reservoir | | | | | | 1 | 1 | 4 | 20 | | |
Competent Cell Sets
Each set contains 0.2 ml of each competent cell strain listed, SOC Medium and Test Plasmid
| (DE3) Competent Cell Set 1
includes BL21(DE3), BLR(DE3), HMS174(DE3), Tuner(DE3), and NovaBlue(DE3) | (DE3) Competent Cell Set 2
includes Rosetta 2(DE3), Rosetta-gami(DE3), , Rosetta-gami B(DE3), RosettaBlue(DE3), Origami(DE3), and Origami B(DE3) | (DE3)pLysS Competent Cell Set 1
includes BL21(DE3)pLysS, BLR(DE3)pLysS, HMS174(DE3)pLysS, and Tuner(DE3)pLysS | (DE3)pLysS Competent Cell Set 2
includes Rosetta 2(DE3)pLysS, Rosetta-gami(DE3)pLysS, Rosetta-gami B(DE3)pLysS, RosettaBlue(DE3)pLysS, Origami(DE3)pLysS, and Origami B(DE3)pLysS | Non-lDE3 Lysogen Competent Cell Set
includes NovaBlue, BL21, Origami B, Rosetta, Rosetta-gami B | | | | HT96 Isothermal Block
holds four Quarters, a full QuarterPack, or one HT96 Competent Cell Plate |
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