The pETcoco™-2 expression vector is designed to allow "on command" amplification of vector copy number from single copy (1–2 plasmid copies per cell) to medium copy (20–50 per cell). The vector is maintained in the single copy state by utiliziing the oriS and repE elements of the F episome together with the parABC partition determinants. The medium copy state is obtained by employing the RK-2 derived trfA replicator acting at oriV. The switch from single copy to medium copy status is achieved by inducing the expression of the trfA gene with arabinose. The dual replicon nature of these vectors promotes enhanced stability of recombinant plasmids and extremely low basal expression levels in the single copy state, while allowing robust target gene expression typical of the pET vectors. pETcoco-2 contains the ampicillin resistance gene. The sequence is numbered relative to the start of T7 transcription at +1.

This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.

Fusion Tags:	His•Tag, S•Tag, HSV•Tag
Vector Family:	pETcoco

Components:

•	10 µg	pETcoco-2 DNA
•	0.2 ml	Induction Control Glycerol Stock