LOCUS pBiEx-3 5373 bp DNA circular 8-JAN-2003
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.informaxinc.com/
COMMENT ORIGDB|GenBank
COMMENT VNTDATE|283014399|
COMMENT VNTDBDATE|291055601|
COMMENT VNTNAME|pBiEx-3|
COMMENT VNTAUTHORNAME|Scott Monsma|
COMMENT VNTAUTHORTEL|608-238-6110|
COMMENT VNTAUTHORFAX|608-238-1388|
COMMENT VNTAUTHOREML|scott.monsma@novagen.com|
COMMENT VNTAUTHORWWW|www.novagen.com|
COMMENT VNTAUTHORAD1|EMD Biosciences, Inc.|
COMMENT VNTREPLTYPE|Plasmid
COMMENT VNTEXTCHREPL|Bacteria
FEATURES Location/Qualifiers
enhancer 1..482
/vntifkey="9"
/label=hr5
promoter 487..1082
/vntifkey="29"
/label=IE1\promoter
CDS 3332..4189
/vntifkey="4"
/label=Ap
rep_origin 4950..4950
/vntifkey="33"
/label=Ori
5'UTR 1034..1034
/vntifkey="52"
/label=Transcription\Start
promoter 1092..1108
/vntifkey="30"
/label=T7\Promoter
protein_bind 1113..1133
/vntifkey="31"
/label=lacO
RBS 1166..1171
/vntifkey="32"
/label=RBS
protein_bind 1284..1328
/vntifkey="31"
/label=S-Tag
misc_binding 1350..1373
/vntifkey="20"
/label=His-Tag
terminator 1434..1481
/vntifkey="43"
/label=T7\Terminator
polyA_signal 1683..1688
/vntifkey="25"
/label=PolyA\signal
misc_feature 1651..1654
/vntifkey="21"
/label=3x\-BseR\I\TCCT\to\CTTC
terminator 1506..1813
/vntifkey="43"
/label=IE1\Terminator
CDS complement(1935..3017)
/vntifkey="4"
/label=lacI
BASE COUNT 1353 a 1314 c 1323 g 1383 t
ORIGIN
1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat
61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca
121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg
181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata
241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac
301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg
361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa
421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac
481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg
541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg
601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag
661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg
721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga
781 tcacgtacgc tcctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta
841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg
901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca
961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt
1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg
1081 accgcggaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct
1141 agaaataatt ttgtttaact ttaagaagga gatataccat ggcatcgtta acacgtcaag
1201 agctcgcgga tcccaattgg cagatctcgg cgcgcctgca ggtcgacggt accggttcga
1261 agcttgcggc cgcatcttct ggtaaagaaa ccgctgctgc gaaatttgaa cgccagcaca
1321 tggactcgcc accgccttct ggcctcgagc accaccatca ccatcaccat cactaagtga
1381 ttaacctcag ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
1441 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatcc
1501 tcaggttata catatatttt gaatttaatt aattatacat atattttata ttatttttgt
1561 cttttattat cgaggggccg ttgttggtgt ggggttttgc atagaaataa caatgggagt
1621 tggcgacgtt gctgcgccaa caccacctcc cttccctcct ttcatcatgt atctgtagat
1681 aaaataaaat attaaaccta aaaacaagac cgcgcctatc aacaaaatga taggcattaa
1741 cttgccgctg acgctgtcac taacgttgga cgatttgccg actaaacctt catcgcccag
1801 taaccaatct agacgtcacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt
1861 tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat
1921 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
1981 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt
2041 cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt
2101 tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt
2161 aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc
2221 gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg
2281 ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga
2341 aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga
2401 gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc
2461 gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta
2521 ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat
2581 aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga
2641 tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag
2701 gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg
2761 cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca
2821 acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa
2881 ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc
2941 tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat
3001 aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc
3061 ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg
3121 cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc
3181 aaggaatggt gcatggacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
3241 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
3301 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
3361 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
3421 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
3481 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
3541 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
3601 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
3661 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
3721 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
3781 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
3841 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
3901 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
3961 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
4021 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
4081 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
4141 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
4201 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
4261 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
4321 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
4381 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
4441 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
4501 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
4561 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
4621 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
4681 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
4741 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
4801 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
4861 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
4921 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
4981 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
5041 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
5101 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
5161 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
5221 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
5281 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
5341 aaacagctat gaccatgatt acgaattccc ggg
//