LOCUS pBiEx-2 6162 bp DNA circular 8-JAN-2003
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.informaxinc.com/
COMMENT ORIGDB|GenBank
COMMENT VNTDATE|284293467|
COMMENT VNTDBDATE|291055562|
COMMENT VNTNAME|pBiEx-2|
COMMENT VNTAUTHORNAME|Scott Monsma|
COMMENT VNTAUTHORTEL|608-238-6110|
COMMENT VNTAUTHORFAX|608-238-1388|
COMMENT VNTAUTHOREML|scott.monsma@novagen.com|
COMMENT VNTAUTHORWWW|www.novagen.com|
COMMENT VNTAUTHORAD1|EMD Biosciences, Inc.|
COMMENT VNTREPLTYPE|Plasmid
COMMENT VNTEXTCHREPL|Bacteria
FEATURES Location/Qualifiers
protein_bind 1959..1976
/vntifkey="31"
/label=Thrombin
protein_bind 1995..2009
/vntifkey="31"
/label=Ek
enhancer 1..482
/vntifkey="9"
/label=hr5
promoter 487..1082
/vntifkey="29"
/label=IE1\promoter
CDS 4121..4978
/vntifkey="4"
/label=Ap
rep_origin 5739..5739
/vntifkey="33"
/label=Ori
5'UTR 1034..1034
/vntifkey="52"
/label=Transcription\Start
promoter 1092..1108
/vntifkey="30"
/label=T7\Promoter
protein_bind 1113..1133
/vntifkey="31"
/label=lacO
RBS 1166..1171
/vntifkey="32"
/label=RBS
misc_binding 1872..1889
/vntifkey="20"
/label=His-Tag
protein_bind 1899..1943
/vntifkey="31"
/label=S-Tag
protein_bind 2118..2153
/vntifkey="31"
/label=HSV-Tag
terminator 2223..2270
/vntifkey="43"
/label=T7\Terminator
polyA_signal 2472..2477
/vntifkey="25"
/label=PolyA\signal
misc_feature 2440..2443
/vntifkey="21"
/label=3x\-BseR\I\TCCT\to\CTTC
terminator 2295..2602
/vntifkey="43"
/label=IE1\Terminator
CDS complement(2724..3806)
/vntifkey="4"
/label=lacI
misc_feature 1179..1841
/vntifkey="21"
/label=GST
BASE COUNT 1576 a 1463 c 1510 g 1613 t
ORIGIN
1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat
61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca
121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg
181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata
241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac
301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg
361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa
421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac
481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg
541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg
601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag
661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg
721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga
781 tcacgtacgc tcctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta
841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg
901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca
961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt
1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg
1081 accgcggaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct
1141 agaaataatt ttgtttaact ttaagaagga gatataccat ggcatcccct atactaggtt
1201 attggaaaat taagggcctt gtgcaaccca ctcgacttct tttggaatat cttgaagaaa
1261 aatatgaaga gcatttgtat gagcgcgatg aaggtgataa atggcgaaac aaaaagtttg
1321 aattgggttt ggagtttccc aatcttcctt attatattga tggtgatgtt aaattaacac
1381 agtctatggc catcatacgt tatatagctg acaagcacaa catgttgggt ggttgtccaa
1441 aagagcgtgc agagatttca atgcttgaag gagcggtttt ggatattaga tacggtgttt
1501 cgagaattgc atatagtaaa gactttgaaa ctctcaaagt tgattttctt agcaagctac
1561 ctgaaatgct gaaaatgttc gaagatcgtt tatgtcataa aacatattta aatggtgatc
1621 atgtaaccca tcctgacttc atgttgtatg acgctcttga tgttgtttta tacatggacc
1681 caatgtgcct ggatgcgttc ccaaaattag tttgttttaa aaaacgtatt gaagctatcc
1741 cacaaattga taagtacttg aaatccagca agtatatagc atggcctttg cagggctggc
1801 aagccacgtt tggtggtggc gaccatcctc caaaatcgga tggttcaact agtggttctg
1861 gttccatggc acaccatcac caccatcact cttctggtaa agaaaccgct gctgcgaaat
1921 ttgaacgcca gcacatggac tcgccaccgc cttctggtct ggtcccccgg ggcagcgcag
1981 gttctggtac gattgatgac gacgacaaga tctctggccc gggctcggat cccaattggg
2041 agctcgtgta cacggcgcgc ctgcaggtcg acggtaccgg ttcgaagctt gcggccgcac
2101 agctgtatac acgtgcaagc cagccagaac tcgccccgga agaccccgag gatctcgagc
2161 actaagtgat taacctcagc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
2221 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
2281 gaactatcct caggttatac atatattttg aatttaatta attatacata tattttatat
2341 tatttttgtc ttttattatc gaggggccgt tgttggtgtg gggttttgca tagaaataac
2401 aatgggagtt ggcgacgttg ctgcgccaac accacctccc ttccctcctt tcatcatgta
2461 tctgtagata aaataaaata ttaaacctaa aaacaagacc gcgcctatca acaaaatgat
2521 aggcattaac ttgccgctga cgctgtcact aacgttggac gatttgccga ctaaaccttc
2581 atcgcccagt aaccaatcta gacgtcacga tagtcatgcc ccgcgcccac cggaaggagc
2641 tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct
2701 aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
2761 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg
2821 gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac cgcctggccc
2881 tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg
2941 atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactacc
3001 gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc
3061 atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg
3121 gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt
3181 tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga gacagaactt
3241 aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc
3301 agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg gtcagagaca
3361 tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc atcctggtca
3421 tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc
3481 gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct ggcacccagt
3541 tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg
3601 gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc cacgcggttg
3661 ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt cgcagaaacg
3721 tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc atactctgcg
3781 acatcgtata acgttactgg tttcacattc accaccctga attgactctc ttccgggcgc
3841 tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc
3901 tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac
3961 cgccgccgca aggaatggtg catggacgtc aggtggcact tttcggggaa atgtgcgcgg
4021 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
4081 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
4141 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
4201 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
4261 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
4321 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
4381 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
4441 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
4501 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
4561 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
4621 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
4681 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
4741 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
4801 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
4861 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
4921 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
4981 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
5041 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
5101 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
5161 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
5221 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
5281 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
5341 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
5401 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
5461 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
5521 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
5581 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
5641 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
5701 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
5761 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
5821 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
5881 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
5941 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
6001 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
6061 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
6121 tcacacagga aacagctatg accatgatta cgaattcccg gg
//