LOCUS pBiEx-1 5475 bp DNA circular 15-JAN-2003
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.informaxinc.com/
COMMENT ORIGDB|GenBank
COMMENT VNTDATE|283014604|
COMMENT VNTDBDATE|291658484|
COMMENT VNTNAME|pBiEx-1|
COMMENT VNTAUTHORNAME|Scott Monsma|
COMMENT VNTAUTHORTEL|608-238-6110|
COMMENT VNTAUTHORFAX|608-238-1388|
COMMENT VNTAUTHOREML|scott.monsma@novagen.com|
COMMENT VNTAUTHORWWW|www.novagen.com|
COMMENT VNTAUTHORAD1|EMD Biosciences, Inc.|
COMMENT VNTREPLTYPE|Plasmid
COMMENT VNTEXTCHREPL|Bacteria
FEATURES Location/Qualifiers
misc_feature 1176..1182
/vntifkey="21"
/label=Kozak\-3\to\+4
protein_bind 1272..1289
/vntifkey="31"
/label=Thrombin
protein_bind 1308..1322
/vntifkey="31"
/label=Ek
enhancer 1..482
/vntifkey="9"
/label=hr5
promoter 487..1082
/vntifkey="29"
/label=IE1\promoter
CDS 3434..4291
/vntifkey="4"
/label=Ap
rep_origin 5052..5052
/vntifkey="33"
/label=Ori
5'UTR 1034..1034
/vntifkey="52"
/label=Transcription\Start
promoter 1092..1108
/vntifkey="30"
/label=T7\Promoter
protein_bind 1113..1133
/vntifkey="31"
/label=lacO
RBS 1166..1171
/vntifkey="32"
/label=RBS
misc_binding 1185..1202
/vntifkey="20"
/label=His-Tag
protein_bind 1212..1256
/vntifkey="31"
/label=S-Tag
protein_bind 1431..1466
/vntifkey="31"
/label=HSV-Tag
terminator 1536..1583
/vntifkey="43"
/label=T7\Terminator
misc_feature 1177..1472
/vntifkey="21"
/label=Multiple\Cloning\Sites
polyA_signal 1785..1790
/vntifkey="25"
/label=PolyA\signal
misc_feature 1753..1756
/vntifkey="21"
/label=3x\-BseR\I\TCCT\to\CTTC
terminator 1608..1915
/vntifkey="43"
/label=IE1\Terminator
CDS 2037..3119
/vntifkey="4"
/label=lacI
BASE COUNT 1373 a 1346 c 1358 g 1398 t
ORIGIN
1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat
61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca
121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg
181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata
241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac
301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg
361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa
421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac
481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg
541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg
601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag
661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg
721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga
781 tcacgtacgc tcctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta
841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg
901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca
961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt
1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg
1081 accgcggaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct
1141 agaaataatt ttgtttaact ttaagaagga gatataccat ggcacaccat caccaccatc
1201 actcttctgg taaagaaacc gctgctgcga aatttgaacg ccagcacatg gactcgccac
1261 cgccttctgg tctggtcccc cggggcagcg caggttctgg tacgattgat gacgacgaca
1321 agatctctgg cccgggctcg gatcccaatt gggagctcgt gtacacggcg cgcctgcagg
1381 tcgacggtac cggttcgaag cttgcggccg cacagctgta tacacgtgca agccagccag
1441 aactcgcccc ggaagacccc gaggatctcg agcactaagt gattaacctc agccgaaagg
1501 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
1561 aacgggtctt gaggggtttt ttgctgaaag gaggaactat cctcaggtta tacatatatt
1621 ttgaatttaa ttaattatac atatatttta tattattttt gtcttttatt atcgaggggc
1681 cgttgttggt gtggggtttt gcatagaaat aacaatggga gttggcgacg ttgctgcgcc
1741 aacaccacct cccttccctc ctttcatcat gtatctgtag ataaaataaa atattaaacc
1801 taaaaacaag accgcgccta tcaacaaaat gataggcatt aacttgccgc tgacgctgtc
1861 actaacgttg gacgatttgc cgactaaacc ttcatcgccc agtaaccaat ctagacgtca
1921 cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca
1981 tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac
2041 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
2101 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg
2161 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
2221 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat
2281 gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg
2341 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
2401 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc
2461 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
2521 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
2581 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa
2641 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg
2701 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca
2761 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt
2821 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg
2881 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac
2941 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc
3001 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa
3061 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca
3121 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
3181 cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa
3241 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatggac
3301 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
3361 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
3421 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
3481 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
3541 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
3601 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
3661 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
3721 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
3781 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
3841 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
3901 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
3961 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
4021 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
4081 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
4141 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
4201 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
4261 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
4321 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
4381 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
4441 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
4501 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
4561 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
4621 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
4681 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
4741 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
4801 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
4861 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
4921 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
4981 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
5041 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
5101 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
5161 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
5221 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
5281 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
5341 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
5401 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
5461 ttacgaattc ccggg
//