LOCUS Gateway_Nova_pET-57-DEST 8362 bp DNA circular 10-SEP-2007
DEFINITION Gateway Nova pET-57-DEST 6xHis/Nus-Tag/Tb/attR1-CamccdB-attR2/Strep-Tag.
SOURCE
ORGANISM
FEATURES Location/Qualifiers
misc_feature 6522..8190
/vntifkey="21"
/label=Region\replaced\by\pENTR\Target
misc_recomb complement(8094..8218)
/vntifkey="86"
/label=attR2
misc_RNA complement(1656..1763)
/vntifkey="53"
/label=RNA\I
misc_RNA 1648..2203
/vntifkey="53"
/label=RNA\II
CDS complement(2634..2825)
/vntifkey="4"
/label=ROP
RBS 4944..4949
/vntifkey="32"
/label=RBS-1
primer 4824..4839
/vntifkey="27"
/label=pET\Upstream\Primer
promoter 4870..4886
/vntifkey="30"
/label=T7\promoter
CDS complement(3397..4479)
/vntifkey="4"
/label=lac\I
rep_origin 2203..2203
/vntifkey="33"
/label=ColE1\pBR322\origin
CDS complement(582..1442)
/vntifkey="4"
/label=bla\(amp)\sequence
rep_origin 13..460
/vntifkey="33"
/label=f1\origin
protein_bind 4890..4911
/vntifkey="31"
/label=lac\operator
terminator 8290..8337
/vntifkey="43"
/label=T7\Terminator
-35_signal 1616..1621
/vntifkey="48"
/label=-35\RNA\II
-10_signal 1639..1644
/vntifkey="47"
/label=-10\RNA\II
misc_RNA 1652..2203
/vntifkey="53"
/label=RNA\II
-35_signal complement(1793..1798)
/vntifkey="48"
/label=-35\RNA\I
-10_signal complement(1769..1774)
/vntifkey="47"
/label=-10\RNA\I
misc_RNA complement(1626..1762)
/vntifkey="53"
/label=RNA\I
terminator 1607..1625
/vntifkey="43"
/label=RNA\I\Terminator
primer complement(8276..8294)
/vntifkey="27"
/label=T7\Terminator\Primer
primer 4870..4889
/vntifkey="27"
/label=T7\Promoter\Primer
protein_bind 4969..4986
/vntifkey="31"
/label=His-Tag
primer complement(4960..4996)
/vntifkey="27"
/label=AS-NT\HisT7\GW-D
protein_bind 6493..6510
/vntifkey="31"
/label=Tb
misc_recomb 6514..6638
/vntifkey="86"
/label=attR1
mutation 7262..7262
/vntifkey="62"
/label=C\to\T\-NcoI
mutation 7966..7966
/vntifkey="62"
/label=C\to\T\-BsaI
CDS 6747..7406
/vntifkey="4"
/label=Cam
CDS 7748..8053
/vntifkey="4"
/label=ccdB
protein_bind 8226..8249
/vntifkey="31"
/label=Strep-Tag\II
primer 8214..8261
/vntifkey="27"
/label=S-CT\Strep\GW
CDS 5002..6483
/vntifkey="4"
/label=Nus-Tag
BASE COUNT 2060 a 2074 c 2173 g 2055 t
ORIGIN
1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttctg gcggcacgat
481 ggcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
541 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
601 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
661 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
721 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
781 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
841 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
901 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
961 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
1021 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
1081 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
1141 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
1201 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
1261 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
1321 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
1381 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
1441 atactcttcc tttttcaatc atgattgaag catttatcag ggttattgtc tcatgagcgg
1501 atacatattt gaatgtattt agaaaaataa acaaataggt catgaccaaa atcccttaac
1561 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
1621 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
1681 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
1741 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
1801 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
1861 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
1921 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
1981 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
2041 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
2101 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
2161 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
2221 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
2281 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
2341 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
2401 attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac
2461 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
2521 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
2581 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
2641 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg
2701 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga
2761 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg
2821 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa
2881 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt
2941 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt
3001 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct
3061 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac
3121 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag
3181 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc
3241 cgccagccta gccgggtcct caacgacagg agcacgatca tgctagtcat gccccgcgcc
3301 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc
3361 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
3421 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
3481 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt
3541 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg
3601 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc
3661 gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat
3721 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt
3781 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc
3841 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc
3901 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag
3961 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt
4021 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat
4081 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag
4141 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac
4201 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg
4261 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg
4321 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt
4381 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc
4441 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact
4501 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg
4561 gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
4621 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
4681 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
4741 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
4801 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc gatctcgatc
4861 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag
4921 aaataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat
4981 catcacagcc atatggctag caacaaagaa attttggctg tagttgaagc cgtatccaat
5041 gaaaaggcgc tacctcgcga gaagattttc gaagcattgg aaagcgcgct ggcgacagca
5101 acaaagaaaa aatatgaaca agagatcgac gtccgcgtac agatcgatcg caaaagcggt
5161 gattttgaca ctttccgtcg ctggttagtt gttgatgaag tcacccagcc gaccaaggaa
5221 atcacccttg aagccgcacg ttatgaagat gaaagcctga acctgggcga ttacgttgaa
5281 gatcagattg agtctgttac ctttgaccgt atcactaccc agacggcaaa acaggttatc
5341 gtgcagaaag tgcgtgaagc cgaacgtgcg atggtggttg atcagttccg tgaacacgaa
5401 ggtgaaatca tcaccggcgt ggtgaaaaaa gtaaaccgcg acaacatctc tctggatctg
5461 ggcaacaacg ctgaagccgt gatcctgcgc gaagatatgc tgccgcgtga aaacttccgc
5521 cctggcgacc gcgttcgtgg cgtgctctat tccgttcgcc cggaagcgcg tggcgcgcaa
5581 ctgttcgtca ctcgttccaa gccggaaatg ctgatcgaac tgttccgtat tgaagtgcca
5641 gaaatcggcg aagaagtgat tgaaattaaa gcagcggctc gcgatccggg ttctcgtgcg
5701 aaaatcgcgg tgaaaaccaa cgataaacgt atcgatccgg taggtgcttg cgtaggtatg
5761 cgtggcgcgc gtgttcaggc ggtgtctact gaactgggtg gcgagcgtat cgatatcgtc
5821 ctgtgggatg ataacccggc gcagttcgtg attaacgcaa tggcaccggc agacgttgct
5881 tctatcgtgg tggatgaaga taaacacacc atggacatcg ccgttgaagc cggtaatctg
5941 gcgcaggcga ttggccgtaa cggtcagaac gtgcgtctgg cttcgcaact gagcggttgg
6001 gaactcaacg tgatgaccgt tgacgacctg caagctaagc atcaggcgga agcgcacgca
6061 gcgatcgaca ccttcaccaa atatctcgac atcgacgaag acttcgcgac tgttctggta
6121 gaagaaggct tctcgacgct ggaagaattg gcctatgtgc cgatgaaaga gctgttggaa
6181 atcgaaggcc ttgatgagcc gaccgttgaa gcactgcgcg agcgtgctaa aaatgcactg
6241 gccaccattg cacaggccca ggaagaaagc ctcggtgata acaaaccggc tgacgatctg
6301 ctgaaccttg aaggggtaga tcgtgatttg gcattcaaac tggccgcccg tggcgtttgt
6361 acgctggaag atctcgccga acagggcatt gatgatctgg ctgatatcga agggttgacc
6421 gacgaaaaag ccggagcact gattatggct gcccgtaata tttgctggtt cggtgacgaa
6481 gcgtctggag ctcttgttcc acgtggttct gtcacaagtt tgtacaaaaa agctgaacga
6541 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
6601 acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag
6661 gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga
6721 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
6781 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
6841 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
6901 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
6961 aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
7021 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
7081 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
7141 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
7201 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
7261 ctatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
7321 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
7381 gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa gccagataac
7441 agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc
7501 gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga
7561 cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc
7621 atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg
7681 atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc
7741 tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
7801 ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag
7861 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga
7921 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtttccg ttatcgggga
7981 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
8041 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg
8101 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
8161 atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttt
8221 ccgcttggag ccacccgcag ttcgaaaagt aagtgattaa cctaggctgc tgccaccgct
8281 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
8341 aaaggaggaa ctatatccgg at
//