LOCUS Gateway_Nova_pET-61-DEST 6846 bp DNA circular 10-SEP-2007
DEFINITION Gateway Nova pET-61-DEST 6xHis/Strep/attR1-Cam(-Nco), ccdB (-Bsa)-attR2/Stop.
SOURCE
ORGANISM
FEATURES Location/Qualifiers
misc_recomb complement(6600..6724)
/vntifkey="86"
/label=attR2
misc_feature 5028..6709
/vntifkey="21"
/label=Region\replaced\by\pENTR\Target
misc_RNA complement(1656..1763)
/vntifkey="53"
/label=RNA\I
misc_RNA 1648..2203
/vntifkey="53"
/label=RNA\II
CDS complement(2634..2825)
/vntifkey="4"
/label=ROP
RBS 4944..4949
/vntifkey="32"
/label=RBS-1
primer 4824..4839
/vntifkey="27"
/label=pET\Upstream\Primer
promoter 4870..4886
/vntifkey="30"
/label=T7\promoter
CDS complement(3397..4479)
/vntifkey="4"
/label=lac\I
rep_origin 2203..2203
/vntifkey="33"
/label=ColE1\pBR322\origin
CDS complement(582..1442)
/vntifkey="4"
/label=bla\(amp)\sequence
rep_origin 13..460
/vntifkey="33"
/label=f1\origin
protein_bind 4890..4911
/vntifkey="31"
/label=lac\operator
terminator 6774..6821
/vntifkey="43"
/label=T7\Terminator
-35_signal 1616..1621
/vntifkey="48"
/label=-35\RNA\II
-10_signal 1639..1644
/vntifkey="47"
/label=-10\RNA\II
misc_RNA 1652..2203
/vntifkey="53"
/label=RNA\II
-35_signal complement(1793..1798)
/vntifkey="48"
/label=-35\RNA\I
-10_signal complement(1769..1774)
/vntifkey="47"
/label=-10\RNA\I
misc_RNA complement(1626..1762)
/vntifkey="53"
/label=RNA\I
terminator 1607..1625
/vntifkey="43"
/label=RNA\I\Terminator
primer complement(6760..6778)
/vntifkey="27"
/label=T7\Terminator\Primer
primer 4870..4889
/vntifkey="27"
/label=T7\Promoter\Primer
misc_binding 4963..4980
/vntifkey="20"
/label=His-Tag
primer 4956..4980
/vntifkey="27"
/label=S-NT\6xHis\GW
protein_bind 4987..5010
/vntifkey="31"
/label=Strep-Tag\II
misc_recomb 5020..5144
/vntifkey="86"
/label=attR1
mutation 5768..5768
/vntifkey="62"
/label=C\to\T\-NcoI
mutation 6472..6472
/vntifkey="62"
/label=C\to\T\-BsaI
CDS 5253..5912
/vntifkey="4"
/label=Cam
CDS 6254..6559
/vntifkey="4"
/label=ccdB
primer 4956..5016
/vntifkey="27"
/label=S-NT\6xHis/Strep\GW
primer 6720..6745
/vntifkey="27"
/label=S-CT\Stop\GW
BASE COUNT 1672 a 1713 c 1737 g 1724 t
ORIGIN
1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttctg gcggcacgat
481 ggcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
541 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
601 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
661 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
721 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
781 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
841 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
901 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
961 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
1021 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
1081 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
1141 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
1201 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
1261 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
1321 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
1381 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
1441 atactcttcc tttttcaatc atgattgaag catttatcag ggttattgtc tcatgagcgg
1501 atacatattt gaatgtattt agaaaaataa acaaataggt catgaccaaa atcccttaac
1561 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
1621 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
1681 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
1741 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
1801 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
1861 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
1921 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
1981 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
2041 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
2101 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
2161 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
2221 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
2281 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
2341 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
2401 attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac
2461 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
2521 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
2581 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
2641 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg
2701 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga
2761 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg
2821 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa
2881 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt
2941 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt
3001 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct
3061 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac
3121 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag
3181 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc
3241 cgccagccta gccgggtcct caacgacagg agcacgatca tgctagtcat gccccgcgcc
3301 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc
3361 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
3421 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
3481 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt
3541 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg
3601 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc
3661 gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat
3721 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt
3781 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc
3841 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc
3901 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag
3961 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt
4021 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat
4081 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag
4141 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac
4201 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg
4261 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg
4321 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt
4381 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc
4441 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact
4501 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg
4561 gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
4621 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
4681 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
4741 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
4801 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc gatctcgatc
4861 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag
4921 aaataatttt gtttaacttt aagaaggaga tataccatgg cacatcacca ccaccatcac
4981 gcaagctgga gccacccgca gttcgaaaag ggtgcagtca caagtttgta caaaaaagct
5041 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
5101 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca
5161 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc
5221 cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
5281 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
5341 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
5401 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
5461 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
5521 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
5581 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
5641 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct
5701 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
5761 ttttcactat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
5821 aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
5881 agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca
5941 gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt
6001 atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga
6061 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc
6121 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag
6181 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac
6241 aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
6301 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct
6361 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
6421 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tttccgttat
6481 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
6541 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc
6601 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
6661 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
6721 tggtgtaagt gattaattga gcagcctagg ctgctgccac cgctgagcaa taactagcat
6781 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
6841 ccggat
//