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PhosphoDetect™ Antibodies for Cell Adhesion/Cytoskeleton
 | | |  PhosphoDetect™ Anti-Paxillin (pSer178) Rabbit pAb, Cat. No. ST1069 Whole cell lysate from NIH3T3 cells (~ 2.4 x 105 cells/lane) that were serum-starved for 16 hours and then grown in suspension (lane 1) or plated on fibronectin (50 mg/ml) for one hour (lane 2) or plated on fibronectin and treated with l phosphatase (lane 3) were analyzed by SDS-PAGE and immunoblotting using PhosphoDetect™ Anti-Paxillin (pSer178) (Cat. No. ST1069) at a 1:1000 dilution. Detection was via chemiluminescence. |
| | |  PhosphoDetect™ Anti-WASP (pSer483/484) Rabbit pAb, Cat. No. ST1087 Detection of human WASP phosphorylated on Ser483/484 by immunoblotting. Whole cell lysate (50 mg) from U937, untreated (lane 1) or treated (lane 2) with l phosphatase. PhosphoDetect™ Anti-WASP (pSer483/484) (Cat. No. ST1087)was used at 1 mg/ml. | | | | Antibodies: Cell Adhesion/Cytoskeleton |
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| PhosphoDetect™ Anti-Cortactin (pTyr421) Rabbit pAb |
IB, IC |
mouse |
CB1000 |
| PhosphoDetect™ Anti-MARCKS (pSer152/156) Rabbit pAb |
DB, IB |
rat |
442710 |
| PhosphoDetect™ Anti-MARCKS (pSer159,163,179) Rabbit pAb |
IB, IF, IH |
human, mouse, rabbit, rat |
442709 |
| PhosphoDetect™ Anti-Paxillin (pSer178) Rabbit pAb |
IB |
human, mouse |
ST1069 |
| PhosphoDetect™ Anti-Paxillin (pTyr118) Rabbit pAb |
IB, IC |
chicken, human |
512880 |
| PhosphoDetect™ Anti-RhoA (pSer188) Rabbit pAb |
IB, IP |
bovine, human |
ST1035 |
| PhosphoDetect™ Anti-VASP (pSer239) Mouse mAb (16C2) |
ELISA, IB, IC, IP |
human, mouse |
676602 |
| PhosphoDetect™ Anti-VASP (pSer157) Mouse mAb (5C6) |
ELISA, IB, IC, IP |
human, mouse |
676604 |
| PhosphoDetect™ Anti-WASP (pSer483/484) Rabbit pAb |
IB |
human |
ST1087 |
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| DB = dot blot • FC = flow cytometry • FFS = free-floating sections • FS = frozen sections • IB = immunoblotting IC = immunocytochemistry • IF = immunofluorescent assay • IH = immunohistochemistry • IP = immunoprecipitation PS = paraffin sections • T = tests | | | |
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