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Heat Shock Proteins: Protecting Cancer Cells
 | | | | | | Depending on the level of stress, injured cells may undergo either necrosis or apoptosis. Under extreme stress conditions, when there is diminution in regulated activation of apoptotic pathways, cells undergo necrosis. At lower stress levels, cells activate their apoptotic machinery. However, at sub-lethal stress levels, cells may attempt to survive and activate a stress response system that includes a rapid induction of heat shock proteins (Hsp). Hsp interact with diverse protein substrates and assist in their folding and in the elimination of any misfolded or damaged molecules. They are transiently expressed during cell cycle to prevent differentiating cells from undergoing apoptosis. Many tumor cells have constitutively elevated levels of Hsp that impart protection against cytotoxic agents, thereby raising the apoptosis threshold of these cells. This abnormal expression of Hsp may lead to multi-drug resistance in aggressively growing tumors. Many Hsp, including Hsp27 and Hsp70, have been shown to block apoptosis. Hsp can also allow cancerous cells to escape the immunosurveillance mediated by death ligands and can render these cells resistant to chemotherapy. Hence, Hsp are fast becoming new targets for therapeutic interventions.
| | | | | | | | | Antibodies |
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| Anti-FKBP59/HSP56 (441-458) Rabbit pAb |
IB |
human, mouse, rabbit |
343250 |
| Anti-GroEL Mouse mAb (9A1/2) |
IB, IP |
E. coli |
CB1007 |
| Anti-Heme Oxygenase-1 (1-261) Rabbit pAb |
FC, IB, IC, IH, IP |
canine, guinea pig, hamster, human, monkey, mouse, rabbit, rat, sheep |
374089 |
| Anti-Heme Oxygenase-1 (1-30) Rabbit pAb |
IB, IH, IP |
canine, hamster, human, monkey, mouse, rat |
374090 |
| Anti-Heme Oxygenase-1 Mouse mAb (HO-1-1) |
IB, IC, IP |
bovine, canine, human, monkey, mouse, rat |
374087 |
| Anti-HSF1 Rabbit pAb |
ELISA, IB, IP |
guinea pig, human, monkey, mouse, porcine, rat |
385580 |
| Anti-Hsp25 Rabbit pAb |
IB, IC, IH, IP |
bovine, canine, guinea pig, hamster, mouse, rat |
385877 |
| Anti-Hsp27 Mouse mAb (EMD-35) |
ELISA, IB, IC, IP |
human |
CA1025 |
| Anti-Hsp27 Mouse mAb (G3.1) |
IC, IH, IP |
human, monkey |
386011 |
| PhosphoDetect™ Anti-Hsp27 (pSer15) (10-21) Rabbit pAb |
IC, NOT IB, PS |
human, rat |
ST1005 |
| PhosphoDetect™ Anti-Hsp27 (pSer82) Mouse mAb (5B9) |
IB |
human |
ST1030 |
| Anti-Hsp27 Rabbit pAb |
ELISA, IB, IC, IP |
human, monkey |
386013 |
| Anti-Hsp27 Rabbit pAb |
IB |
human |
CA1015 |
| Anti-Hsp40 Rabbit pAb |
IB, IP |
bovine, canine, chicken, fish, guinea pig, hamster, human, monkey, mouse, porcine, rat, sheep, Xenopus |
386020 |
| Anti-Hsp47 Mouse mAb (M16.10A1) |
EIA, ELISA, IB, IC, IF, IP |
bovine, canine, chicken, guinea pig, hamster, human, monkey, mouse, porcine, rat, sheep |
386023 |
| Anti-Hsp60 Mouse mAb (LK-1) |
EIA, ELISA, FC, IB, IC, IH, IP |
bovine, canine, Drosophila, fish, guinea pig, hamster, human, monkey, mouse, porcine, rabbit, rat, yeast |
386028 |
| Anti-Hsp60 Mouse mAb (LK-2) |
ELISA, IB, IC |
a broad range of species |
386029 |
| Anti-Hsp70 Mouse mAb (C92F3A-5) |
ELISA, FC, FS, IB, IC, IP, PS |
a broad range of species |
386032 |
| Anti-Hsp70 Rabbit pAb |
ELISA, IB, IC, IP |
a broad range of species |
386035 |
| Anti-Hsp70/Hsc70 Mouse mAb (N27F3-4) |
FC, IB, IH, IP |
a broad range of species |
386037 |
| Anti-Hsp72/73 (Ab-1) Mouse mAb (W27) |
IB, IF, IP, PS |
human, mouse, rat |
HSP01 |
| Anti-Hsp90 Mouse mAb (AC88) |
IB, IC, IP |
a broad range of species |
386040 |
| Anti-Hsp90 Rat mAb (16F1) |
ELISA, IB, IC, IH, IP |
a broad range of species |
386041 |
| Anti-Hsp90a Rabbit pAb |
IB |
human |
CA1016 |
| Anti-Hsp90a Rat mAb (9D2) |
IB, IC, IP |
human |
386043 |
| Anti-Hsp90a Mouse mAb (EMD-17D7) |
ELISA, IB, IP |
human |
CA1023 |
| Anti-Hsp90b Mouse mAb (EMD-5E12) |
ELISA, IB, IC, IP |
human |
CA1024 |
| Anti-Hsp90b Rabbit pAb |
IB |
human |
CA1017 |
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| | | | Kits |
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| PhosphoDetect™ Hsp27 (pSer78/82) ELISA Kit |
96-well plate |
96 Tests |
0.1-10 ng/ml |
4.5 h |
Cell lysates |
CBA078 |
| Hsp27 ELISA Kit |
96-well plate |
96 Tests |
0.02-1 ng/ml |
3.5 h |
Cell lysates, plasma, serum, tissue extracts |
QIA119 |
| Hsp90a ELISA Kit |
96-well plate |
96 Tests |
0.3 - 20 ng/ml |
4 h |
Cell lysates, tissue extracts and biological fluids |
CBA057 |
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| | | | Inhibitors | |
| | | | Proteins |
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| Heme Oxygenase-1, Rat, Recombinant, E. coli |
374093 |
| Hsc70, Bovine, Recombinant, E. coli |
385873 |
| Hsp27, His·Tag®, Human, Recombinant, S. frugiperda |
385909 |
| Hsp27, Human, Recombinant, E. coli |
385891 |
| Hsp40, Human, Recombinant, E. coli |
385892 |
| Hsp60, Human, Recombinant, E. coli |
385894 |
| Hsp65, Mycobacterium bovis BCG, Recombinant, E. coli |
385895 |
| Hsp70, Human, Recombinant, E. coli |
385896 |
| Hsp90, Bovine |
385897 |
| Hsp90, Human |
385898 |
| Hsp90b, His·Tag®, Human, Recombinant, S. frugiperda |
385903 |
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