EMD - Calbiochem - Inhibitors: Cocktails

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Protease and Phosphatase Inhibitor Cocktails

Inhibitor Cocktails

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Protease Inhibitors | Phosphatase Inhibitors

Try our convenient inhibitor cocktails to knock out a broad range of proteolytic activities. Information is provided regarding the recommended use, target protease class and the effective concentration range.

Protease and Phosphatase Inhibitor Cocktails Brochure
	Perfect tools for scientists who are involved in protein sample preparation, enzyme purification, and other proteomic applications. View PDF or request a free copy

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Protease Inhibitor Cocktails

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Protease Inhibitor Cocktail Set I, Cat. No. 539131

A cocktail containing five protease inhibitors that will inhibit a broad range of proteases. Reconstitute each vial with 1 ml H₂O to obtain a 100x stock solution. When diluted, 1x stock solution contains the following amount of inhibitors.

Product	Cat. No.	Mol. Wt.	1x Concentration	Target Protease
AEBSF, Hydrochloride	101500	239.5	500 mM	Serine Proteases
Aprotinin	616398	6512	150 nM	Serine Proteases and Esterases
E-64 Protease Inhibitor	324890	357.4	1 mM	Cysteine Proteases
EDTA, Disodium	324503	372.2	0.5 mM	Metalloproteases
Leupeptin, Hemisulfate	108975	475.6	1 mM	Cysteine Proteases and Trypsin-like proteases

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Protease Inhibitor Cocktail Set I, Animal-Free, Cat. No. 535142

A cocktail of four protease inhibitors for the inhibition of serine and cysteine proteases, but not
metalloproteases. Contains aprotinin that is free of animal products. 1X solution will contain the
following protease inhibitors:

Product	Cat. No.	Mol. Wt.	1x Concentration	Target Protease
AEBSF, Hydrochloride	101500	239.5	500 mM	Serine Proteases
Aprotinin, Bovine, Recombinant, Nicotiana sp., Animal-Free	616371	6509-6515	150 nM	Serine Proteases and Esterases
E-64 Protease Inhibitor	324890	357.4	1 mM	Cysteine Proteases
EDTA, Disodium	324503	372.2	0.5 mM	Metalloproteases
Leupeptin, Hemisulfate	108975	475.6	1 mM	Cysteine Proteases and Trypsin-like proteases

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Protease Inhibitor Cocktail Set II, Cat. No. 539132

A cocktail of five protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, serine, and metalloproteases as well as aminopeptidases. This cocktail is recommended for use with bacterial cell extracts. 1X solution will contain the following protease inhibitors:

Product	Cat. No.	Mol. Wt.	1x Concentration	Target Protease
AEBSF, Hydrochloride	101500	239.5	20 mM	Serine Proteases
Bestatin	200484	308.4	1.7 mM	Aminopeptidase B and Leucine Aminopeptidase
E-64 Protease Inhibitor	324890	357.4	200 mM	Cysteine Proteases
EDTA, Disodium	324503	372.2	85 mM	Metalloproteases
Pepstatin A	N/A	685.9	2 mM	Aspartic Proteases

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Protease Inhibitor Cocktail Set III, Cat. No. 539134

This cocktail is recommended for use with mammalian cells and tissue extracts. Cocktail contains six protease inhibitors (in 1 ml of DMSO) with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases. Each vial contains the following amount of inhibitors. One ml is sufficient for 20 g of tissue.

Product	Cat. No.	Mol. Wt.	Concentration in the Vial	Target Protease
AEBSF, Hydrochloride	101500	239.5	100 mM	Serine Proteases
Aprotinin, Bovine Lung, Lyophilized	616398	6512	80 mM	Broad Spectrum, Serine Proteases
Bestatin	200484	308.4	5 mM	Aminopeptidase B and Leucine Aminopeptidase
E-64 Protease Inhibitor	324890	357.4	1.5 mM	Cysteine Proteases
Leupeptin, Hemisulfate	108975	475.6	2 mM	Cysteine Proteases and Trypsin-like Proteases
Pepstatin A	N/A	685.9	1 mM	Aspartic Proteases

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Protease Inhibitor Cocktail Set III, Animal-Free, Cat. No. 535140

A cocktail of six protease inhibitors with broad specificity for the inhibition of aspartic, cysteine,
serine, and aminopeptidases. Contains aprotinin that is free of animal products. This cocktail is
recommended for use with mammalian cell and tissue extracts. Each vial contains the following
protease inhibitors:

Product	Cat. No.	Mol. Wt.	Concentration in the Vial	Target Protease
AEBSF, Hydrochloride	101500	239.5	100 mM	Serine Proteases
Aprotinin, Bovine, Recombinant, Nicotiana sp., Animal-Free	616371	6509- 6515	80 mM	Broad Spectrum, Serine Proteases
Bestatin	200484	308.4	5 mM	Aminopeptidase B and Leucine Aminopeptidase
E-64 Protease Inhibitor	324890	357.4	1.5 mM	Cysteine Proteases
Leupeptin, Hemisulfate	108975	475.6	2 mM	Cysteine Proteases and Trypsin-like Proteases
Pepstatin A	N/A	685.9	1 mM	Aspartic Proteases

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Protease Inhibitor Cocktail Set IV, Cat. No. 539136

This cocktail is recommended for fungal and yeast cell extracts. Cocktail contains four protease inhibitors (in 1 ml of DMSO) with broad specificity for the inhibition of aspartic-, cysteine-, metallo-, and serine-proteases. Each vial contains the following amount of inhibitors.

Product	Cat. No.	Mol. Wt.	Concentration in the Vial	Target Protease
AEBSF, Hydrochloride	101500	239.5	100 mM	Serine Proteases
E-64 Protease Inhibitor	324890	357.4	1.5 mM	Cysteine Proteases
Pepstatin A	N/A	685.9	2 mM	Aspartic Proteases
o-Phenanthroline	516705	198.2	500 mM	Metalloproteases

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Protease Inhibitor Cocktail Set V, EDTA-Free, Cat. No. 539137

A cocktail containing four protease inhibitors for the inhibition of serine, cysteine, but not metalloproteases. Reconstitute each vial with 1 ml H₂O to obtain a 100x stock solution. When diluted to 1x stock solution, the set will contain the following amount of inhibitors.

Product	Cat. No.	Mol. Wt.	1x Concentration	Target Protease
AEBSF, Hydrochloride	101500	239.5	500 mM	Serine Proteases
Aprotinin, Bovine Lung, Crystalline	616370	6512	150n M	Serine Proteases and Esterases
E-64 Protease Inhibitor	324890	357.4	1 mM	Cysteine Proteases
Leupeptin, Hemisulfate	108975	475.6	1 mM	Cysteine Proteases and Trypsin-like Proteases

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Protease Inhibitor Cocktail Set V, Animal-Free, Cat. No. 535141

A cocktail of four protease inhibitors for the inhibition of serine and cysteine proteases, but not
metalloproteases. Contains aprotinin that is free of animal products. This cocktail is recommended
for use with mammalian cell and tissue extracts. 1X solution will contain the following protease
inhibitors:

Product	Cat. No.	Mol. Wt.	1x Concentration	Target Protease
AEBSF, Hydrochloride	101500	239.5	500 mM	Serine Proteases
Aprotinin, Bovine, Recombinant, Nicotiana sp., Animal-Free	616371	6509- 6515	150 nM	Broad Spectrum, Serine Proteases
E-64 Protease Inhibitor	324890	357.4	1 mM	Cysteine Proteases
Leupeptin, Hemisulfate	108975	475.6	1 mM	Cysteine Proteases and Trypsin-like Proteases

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Protease Inhibitor Cocktail Set VI, Cat. No. 539133

This cocktail is recommended for use with plant cell extracts. Cocktail contains six protease inhibitors (in 1 ml DMSO) with broad specificity for the inhibition of aspartic, cysteine, serine, and metalloproteases as well as aminopeptidases. Each vial contains the following amount of inhibitors. One ml is recommended for 30 g various plant tissues.

Product	Cat. No.	Mol. Wt.	Concentration in the Vial	Target Protease
AEBSF, Hydrochloride	101500	239.5	200 mM	Serine Proteases
Bestatin	200484	308.4	10 mM	Aminopeptidase B and Leucine Aminopeptidase
E-64 Protease Inhibitor	324890	357.4	3 mM	Cysteine Proteases
Leupeptin, Hemisulfate	108975	475.6	2 mM	Cysteine Proteases and Trypsin-like Proteases
o-Phenanthroline	516705	198.2	500 mM	Metalloproteases
Pepstatin A	N/A	685.9	2 mM	Aspartic Proteases

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Protease Inhibitor Cocktail Set VII, Cat. No. 539138

This cocktail is recommended for purification of proteins containing His•Tag� sequences. Cocktail contains five protease inhibitors (in 1 ml DMSO) with broad specificity for the inhibition of cysteine, serine, aspartic, and thermolysin-like proteases and aminopeptidases. Each vial contains the following amount of inhibitors. One ml is recommended for the inhibition of proteases in 10 g cells.

Product	Cat. No.	Mol. Wt.	Concentration in the Vial	Target Protease
AEBSF, Hydrochloride	101500	239.5	100 mM	Serine Proteases
Bestatin	200484	308.4	5 mM	Aminopeptidase B and Leucine Aminopeptidase
E-64 Protease Inhibitor	324890	357.4	1.5 mM	Cysteine Proteases
Pepstatin A	N/A	685.9	2 mM	Aspartic Proteases
Phosphoramidon	525276	587.5	0.2 mM	Metalloendopeptidases

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Protease Inhibitor Cocktail Set VIII, Cat. No. 539129

A DMSO solution of three protease inhibitors with selective specificity for the inhibition of cysteine proteases, including calpains, cathepsins, and papain. Each vial contains the following amount of inhibitors.
Product
Cat. No.

Mol. Wt.

Concentration in the Vial

Target Protease

ALLN
208719
383.5�
1.56 mM
Cysteine Proteases

E-64 Protease Inhibitor
324890
357.4
1.5 mM
Cysteine Proteases

Cathepsin Inhibitor I
219415
475.5
500 mM
Cysteine Proteases

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Serine Protease Inhibitor Cocktail Set I, Cat. No. 565000

A cocktail of four protease inhibitors that is useful for inhibition of a broad range of serine proteases. Reconstitute each vial with 1 ml of H₂O to obtain a 100x stock solution. Each vial contains the following amount of inhibitors.
Product
Cat. No.

Mol. Wt.

Concentration in the Vial

Target Protease

AEBSF, Hydrochloride
101500
239.5
500 mM
Serine Proteases

Aprotinin
616370
6512
150n M
Serine Proteases and
Esterases

Elastatinal
324691
512.6�
20 mM
Serine Proteases

GGACK
347436
392.8
1 mM
Serine Proteases

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Phosphatase Inhibitor Cocktails

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Phosphatase Inhibitor Cocktail Set I, Cat. No. 524624

A cocktail of three inhibitors of alkaline phosphatases and serine/threonine protein phosphatases such as PP1 and PP2A. Each vial contains 2.5 mM (-)-p-Bromotetramisole Oxalate, 500 mM Cantharidin (Cat. No. 210155), and 500 nM Microcystin-LR (Cat. No. 475815). Note: 1 set = 5 x 1 ml.
Product
Cat. No.

Mol. Wt.

Concentration in the Vial

Target Phosphatase

(-)-p-
Bromotetramisole Oxalate
N/A
373.23
2.5 mM
Alkaline Phosphatase

Cantharidin
210155
196.2�
500 mM
Serine/Threonine Phosphatases

Microcystin-LR, Microcystis aeruginosa
475815
995.2
500 nM
Serine/Threonine Phosphatases

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Phosphatase Inhibitor Cocktail Set II, Cat. No. 524625

A cocktail of five inhibitors of acid and alkaline phosphatases as well as protein tyrosine phosphatases. Suitable for use with tissue and cell extracts, including extracts containing detergents. Dilute 1:100 just prior to use. Note: 1 set = 5 x 1 ml. Each vial contains:
Product
Cat. No.

Mol. Wt.

Concentration in the Vial

Target Phosphatase

Imidazole
4015
68.1
200 mM
Alkaline Phosphatase

Sodium Fluoride
N/A
42.0
100 mM
Acid Phosphatase

Sodium Molybdate
N/A
205.9
115 mM
Acid Phosphatase

Sodium Orthovanadate
567540
183.9
100 mM
Protein Tyrosine Phosphatase, Alkaline Phosphatase

Sodium Tartrate Dihydrate
N/A
230.1
400 mM
Acid Phosphatase

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Phosphatase Inhibitor Cocktail Set III, Cat. No. 524627

A cocktail of four phosphatase inhibitors for broadspectrum inhibition of both serine/threonine and protein tyrosine phosphatases. Available as a 1 ml vial or as a set of five 1 ml vials. Each vial contains 1 ml aqueous solution of the phosphatase inhibitor cocktail. The concentrations of the individual inhibitors are shown in the table below.

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Product	Mol. Wt.	Concentration in the Vial	Target Phosphatases
Sodium Fluoride	42.0	50 mM	Acid phosphatases
Sodium Orthovanadate	183.9	1 mM	Protein tyrosine phosphatase, Alkaline phosphatase
Sodium Pyrophosphate, decahydrate	416.1	10 mM	PP1 and PP2A
b-Glycerophosphate	306.1	10 mM	Ser/Thr phosphatases

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Phosphatase Inhibitor Cocktail Set IV, Cat. No. 524628

A cocktail of three phosphatase inhibitors for the inhibition of both serine/threonine and alkaline phosphatases. Available as a 1 ml vial or as a set of five 1 ml vials. Each vial contains a 1 ml solution phosphatase inhibitor cocktail. The concentrations of the individual inhibitors are shown in the table below.

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Product	Mol. Wt.	Concentration in the Vial	Target Phosphatases
(-) -p-Bromotetramisole oxalate	373.2	2.5 mM	Alkaline phosphatases
Cantharidin	196.2	500 mM	PP1 and PP2A
Calyculin A, Discodermia calyx	1009.2	1 mM	PP1 and PP2A

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NEW! Phosphatase Inhibitor Cocktail Set V, 50X, Cat. No. 524629

A cocktail of four phosphatase inhibitors for the inhibition of both serine/threonine and protein tyrosine phosphatases. Available as 1 ml or as a set of five 1 ml vials. Each vial contains 1 ml of aqueous solution with the following components: 250 mM Sodium Fluoride, 50 mM b-Glycerophosphate (Cat. No. 35675), 50 mM Sodium Pyrophosphate Decahydrate, 50 mM Sodium Orthovanadate. The concentrations of the individual inhibitors are shown in the table below.

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Product	Cat. No.	Mol. Wt.	Concentration in the Vial	Target Phosphatases
Sodium Fluoride	N/A	41.99	250 mM	Acid Phosphatases
Sodium Orthovanadate	567540	183.9	50 mM	Protein Tyrosine Phosphatase, Alkaline Phosphatase
Sodium Pyrophosphate, Decahydrate	N/A	446.06	50 mM	Ser/Thr Phosphatases
b-Glycerophosphate	35675	306.1	50 mM	Ser/Thr Phosphatases

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