EMD: InnoCyte 96-well Cell Adhesion Array

Convenient: 96-well plate format; allows cell attachment profiling of various cell lines at the same time
Sensitive: Cell-permeable fluorescent dye; no cell permeabilization needed
Easy to use: Does not require tedious staining method

Determine relative cell attachment to 6 different extracellular matrix proteins!

The ability of cells to adhere to each other or to constituents of the extracellular matrix (ECM) is
important in normal cellular function in mammals. The InnoCyte ECM Cell Adhesion Array, is
designed for the determination of the relative attachment of adherent cell lines to 6 different
extracellular matrix (ECM) proteins: laminin, fibronectin, vitronectin, and collagens type I, type III, and type IV, for evaluation of cell adhesion receptors, and for screening cell adhesion antagonists.

Assay Features:

Inhibition of HT-1080 Cell Attachment to Laminin I. Approximately 10,000 cells were added to wells coated with laminin I in the presence or absence of inhibitory antibody, anti-integrin b1 (Cat. No. CP26). The cells were incubated and labeled and fluorescence was measured as outlined in Figure 1.

Fig. 2

Relative Attachment to Collagens. Approximately 30,000 cells were added to wells containing collagen I, III, or IV, and incubated for 1.5 h at 37°C in a cell culture incubator in the presence of 6% CO₂. Cells were washed gently with D-PBS and labeled with Calcein-AM for 1 h at 37°C in a cell culture incubator in the presence of 6% CO₂. Fluorescence was measured as outlined in the Detailed Protocol.

Fig.3

Relative Cell Attachment of Human Cell Lines. Approximately 30,000 cells were added to wells containing fibronectin, laminin I, collagen type IV, vitronectin, basement membrane complex, and BSA and incubated for 1.5 h at 37°C in a cell culture incubator in the presence of 6% CO₂. Cells were washed gently with D-PBS and labeled with Calcein-AM for 1 h at 37°C in a cell culture incubator in the presence of 6% CO₂. Fluorescence was measured as outlined in the Detailed Protocol.