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InnoCyte™ Cell Adhesion Assay
 | | | | Galectins are a family of carbohydrate binding adhesion molecules (lectins) with affinity for lactose and other b -galactosides. Galectins are characterized by Ca2+ independence and extensive sequence identity in the carbohydrate recognition domain. Although galectins lack a signal peptide and are normally found in the cytosol, they can be externalized by an atypical secretory mechanism. Galectin-1 is a monomeric or homodimeric prototype galectin that is expressed in a variety of cells. Galectin-1 is involved in multiple biological functions, including cell adhesion, apoptosis, and tumor metastasis. Galectin-1 can modulate cell-cell as well as cell-matrix interactions. Galectin-3 is expressed in normal and neoplastic cells and regulates cell growth, cell adhesion, differentiation, and cell death. There is evidence to suggest that galectin-3 plays an important role in tumor progression and metastasis. Recombinant galectin-3 is reported to bind a1b1 integrin, a receptor for laminin and collagen. Galectin-3 is also a substrate for MMP-2 and MMP-9 cleavage, resulting in a 22 kDa fragment containing a carbohydrate recognition domain and 9 kDa fragment containing the N-terminal part of galectin-3. | | | | InnoCyte™ ECM Cell Adhesion Assay, Galectin-1/Galectin-3 (Cat. No. CBA026) | | The InnoCyte™ Cell Adhesion Assay, Galectin-1/Galectin-3 is designed for the determination of the relative attachment of adherent cell lines to galectin-1 and galectin-3, for evaluation of cell adhesion receptors, and for screening cell adhesion antagonists. | | | | Assay Features: | | | • | 96-well tissue culture plate coated with Galectin-1/Galectin-3 | • | Assay is quick-can be performed in 2.5 hr | • | Adherent cells are labeled with calcein-AM | • | Relative cell attachment is assessed using a fluorescence plate reader |
| | | | | | | | | | Relative cell attachment of various cell lines to galectin-1, galectin-3 and BSA. Approximately 40,000 cells were added to wells coated with galectin-1, galectin-3, or BSA and incubated for 1.5h at 37°C in the presence of 6% CO2. Cells were washed gently with D-PBS and labeled with Calcein-AM (Cat. No. 206700) for 1 h at 37°C in the presence of 6% CO2. HT-1080 cells displayed appreciable binding to poly-L-lysine, which served as a positive control (data not shown). Data presented as relative fluorescence units (RFU). | | | | |
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