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Cell Cycle-Cell Proliferation Assays
 
Cell proliferation and the assessment of substances that either promote or inhibit cell proliferation are crucial to the study of cell biology and to drug-discovery research. Several methods have been developed for the sensitive detection of cell proliferation. One common technique is the detection of bromodeoxyuridine (BrdU) incorporation into newly synthesized DNA strands of actively proliferating cells. Other kits measure protein or enzymatic activity markers of cell proliferation. In addition kits to quantitate live, dead, or senescent cells are available. These assays can be used to measure cell proliferation in response to growth factors or cytokines, or to assess cytotoxic compounds.
 
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BrdU Cell Proliferation Assay 96-well plate 200 or 1000 Tests Colorimetric 3 h Intact Cells QIA58
BrdU Cell Proliferation Assay, HTS 96-well plate 200 or 1000 Tests Fluorometric 3 h Intact Cells HTS01
BrdU Immunohistochemistry System 50 Tests Microscopy ~2.5 hours plus labeling time Paraffin-embedded tissues or fixed cells HCS30
Cell Death Detection (Nuclear Matrix Protein) ELISA Kit 96-well plate 96 Tests Colorimetric 4 h Tissue culture supernatant QIA20
Ultrasensitive Cell Proliferation Assay Kit, Fluorogenic 96-well plate 500 Tests Fluorometric 1 h Viable cells QIA128
Live/Dead Double Staining Kit Fluorescence microscopy 100 Tests Fluorescence 0.5 h Cell suspensions or adherent cells QIA76
p21WAF1 ELISA Kit 96-well plate 96 Tests Colorimetric 4 h Cell extracts or tissue culture media QIA18
PCNA ELISA Kit 96-well strip plate 96 Tests Colorimetric 3.5 h Cell lysates, tissue culture media , serum, or plasma QIA59
Rapid Cell Proliferation Kit 96-well plate 500 Tests Colorimetric 0.5 - 4 h Cultured cells QIA127
Senescence Detection Kit 12-well plate 250 Tests Colorimetric 30 min. plus overnight incubation Intact cells QIA117
 
 
 
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are brands of EMD Chemicals Inc., an Affiliate of Merck KGaA, Darmstadt, Germany.