Antibodies for Immunohistochemistry| Cat. No. | Product Name | Product Description |
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| 171598 | Anti-Amyloid Precursor Protein, N-Terminal (44–63), Human (Goat) | A synthetic peptide was used as the immunogen which corresponded to amino acid residues 44–63 of the N-terminus of the human Alzheimer's amyloid precursor protein (APP). This protein is a large membrane protein whose N-terminus projects into the extracellular space. In Alzheimer's disease, APP is broken down to release the 42 amino acid b-amyloid peptide that is found in senile plaques and vessels. Liquid. Polyclonal IgG, undiluted serum. | | Anti-Amyloid Precursor Protein, C-Terminal (751–770), Human (Rabbit) | Immunogen used was a synthetic peptide [(C)KMQQNGYENPTYKFFEQMQN] corresponding to amino acid residues 751–770 of the C-terminal 20 residues of human Alzheimer's amyloid precursor protein (APP). Detects C-terminal soluble products CTF g (~6 kDa), CTF a (~9 kDa), CTF b (~11 kDa), and full-length APP. Recognizes less than 0.1 fmol (0.6 pg) of synthetic C-terminal soluble product of g-secretase cleavage (CTF g-57) by Western blot analysis. May be useful for a novel g-secretase assay based on the detection of CTF g. Liquid. Polyclonal IgG, undiluted serum. | | Anti-Amyloid Precursor Protein, Frameshift Mutant, C-Terminal (339–348), Human (Goat) | Immunogen used was a synthetic peptide corresponding to a distinct region of a frameshift mutant with a GA deletion, resulting in a truncated 45 kDa APP protein with a unique C-terminus. Liquid. Polyclonal IgG, undiluted serum. | | Anti-Amyloid Precursor Protein, Human (Mouse) | Immunogen used was a synthetic peptide (LEVPTDGNAGLLAEPQIAMFC) corresponding to amino acid residues 18–38 of amyloid precursor protein (APP 695). Lyophilized solid. Monoclonal IgG1, purified. Clone 3E9 | | Anti-b-Amyloid Precursor-Like Protein 2, Full-length, Mouse (Rabbit) | Immunogen used was the full-length mouse APLP2. Recognizes specifically APLP2. Does not react with APP and APLP1. Liquid. Polyclonal IgG, undiluted serum. | | Anti-b-Amyloid (Asp-1) (FCA18), Human (Rabbit) | Immunogen used was a synthetic peptide corresponding to the N-terminus of the b-amyloid peptide (Ab). Reported to interact only with the first free aspartyl- residue and recognizes N-terminus part of Ab1-x . Does not recognize -aspartyl- residues in full-length APP or N-acetylated aspartyl- or aspartyl-1 deleted A b peptides.
Ab (Asp-1) is conserved between species. Liquid. Polyclonal IgG | | NE1002 | Anti-b-Amyloid (17–24), Human (Mouse) | Immunogen used was a synthetic peptide corresponding to amino acid residues 17–24 of b-amyloid. Liquid. Mouse monoclonal, IgG2b | | Anti-b-Amyloid (1–17), Human (Mouse) | Immunogen used was a synthetic peptide corresponding to amino acid residues 1–17 of b-amyloid peptide. Liquid. Mouse monoclonal, IgG1 | Unique
offering 171608 | Anti-b-Amyloid 40 (FCA3340), Human (Rabbit) | Immunogen used was a synthetic peptide [(C)GLMVGGVV] corresponding to amino acid residues 704–711 of APP. Specifically recognizes Ab40 and p3-related fragments, and does not recognize APP. Does not cross-react with Ab42 and Ab43 . Liquid. Polyclonal IgG, purified. | Unique
offering 171609 | Anti-b-Amyloid42 (FCA3542), Human (Rabbit) | Immunogen used was a synthetic peptide [(C)MVGGVVIA] corresponding to amino acid residues 706–713 of APP. Specifically recognizes Ab42 and p3-related fragments. Does not recognize APP and does not cross-react with Ab40 or Ab43. Liquid. Polyclonal IgG, purified. | | Anti-Pan b-Amyloid, Human (Rabbit) | Immunogen used was a synthetic peptide corresponding to the b-amyloid peptide (1–40). Recognizes all three b-amyloid peptides, Ab40, Ab42, and Ab43. This antibody is not blocked by b-amyloid peptide (1–12). Liquid. Polyclonal IgG, purified |
| | | Antibody to BACE: b-Amyloid Precursor Protein Cleaving Enzyme | | | Cat. No. | Product Name | Product Description |
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| PC478 | Anti-BACE (Ab-1), Human (Rabbit), BACE: b-Amyloid Precursor Protein Cleaving Enzyme
| Immunogen used was a peptide corresponding to amino acid residues 44–59 of human BACE. BACE is a membrane associated aspartyl protease that is involved in the two step cleavage of amyloid b-peptide and is involved in the development of Alzheimer's disease. Liquid. Polyclonal IgG | | Anti-BACE1, C-Terminal (485–501), Human (Rabbit). BACE: b-Amyloid Precursor Protein Cleaving Enzyme | Immunogen used was a synthetic peptide (CLRQQHDDFADDISLLK) corresponding to amino acid residues 485–501 at the C-terminus of BACE1 (b-site amyloid precursor protein cleaving enzyme 1), coupled to Keyhole Limpet Hemocyanin (KLH). Recognizes both the immature and mature forms of BACE1 protein. Liquid. Polyclonal IgG, undiluted serum |
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| | | Antibody to Apolipoprotein E | |
| | Cat. No. | Product Name | Product Description |
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| Anti-Apolipoprotein E, Human (Mouse) | Immunogen used was a synthetic peptide corresponding to amino acid residues surrounding the polymorphic amino acid position 158 of ApoE. It has been reported that genetic inheritance of apolipoprotein isoform E4 is a significant risk factor for late onset Alzheimer's disease (AD). Detects the E2, E3, and E4 isoforms of apolipoprotein E. Liquid. Mouse monoclonal, IgG1. |
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| PC361 | Anti-Presenilin 1 (14–33) (Ab-3), Human (Goat) | Immunogen used was a synthetic peptide (AQMSEDNHLSNTVRSQNDNR) corresponding to amino acid residues 14–33 from the N-terminal domain of human presenilin 1 (PS 1) protein. Presenilin 1 undergoes proteolytic cleavage in vivo . Liquid. Polyclonal IgG |
| | | | AntigenPlus™ Antigen Retrieval Buffers | |
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Because paraformaldehyde- or formalin-fixed tissues occasionally show low reactivity to antibodies used for immunohistochemistry (IHC), it is advantageous to identify a set of conditions under which the protein structure and reactivity to antibodies can be recovered. This process is called antigen retrieval (AR). Although the specific mechanism of action for AR is unclear, it is possible that some modification of the cross linkages (loosening or breakage) or renaturation of the protein epitopes occurs. The conditions for effective AR are usually determined somewhat empirically and vary depending on the tissue source and antigen-antibody combination. Pretreatment of fixed tissues with an antigen retrieval buffer can dramatically improve antibody staining. For tissues and antibodies for which antigen retrieval is necessary, Novagen has developed the AntigenPlus™ Buffer Set designed for antigen retrieval applications in fixed, paraffin embedded tissues. The Buffer Set consists of three different 5X AntigenPlus Buffers (pH 6, pH 7.4, and pH 10) that can be tested with the tissues of interest. Below are some examples of the striking effects antigen retrieval can have with immunohistochemical staining. See inNovations 17 article Improved Immunohistochemical Staining of Fixed Tissues for more infomation. | Cat. No. | Product Name | Product Description |
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| AntigenPlus™ Buffer Set | The Buffer Set consists of three different 5X AntigenPlus Buffers (pH 6, pH 7.4 and pH 10) that can be tested with the tissues of interest.
Components:
• 100 ml of 5X AntigenPlus Buffer (pH 6)
• 100 ml of 5X AntigenPlus Buffer (pH 7.4)
• 100 ml of 5X AntigenPlus Buffer (pH 10) | | AntigenPlus™ Buffer (pH 6) | 100 ml of 5x AntigenPlus Buffer (pH 6) | | AntigenPlus™ Buffer (pH 7.4) | 100 ml of AntigenPlus Buffer (pH 7.4) | | AntigenPlus™ Buffer (pH 10) | 100 ml of AntigenPlus Buffer (pH 10) |
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