Novagen Duet Vector Table

		Technical Resources

		Technical Information

		Novagen Information

		Vector Tables

		BlueStar Vectors
		Duet Vectors
		Gateway� Nova DEST Vectors
		LIC Duet Adaptors
		M13mp18 Vector
		pBAC Vectors
		pBiEx Vectors
		pCDF & pRSF Vectors
		pCITE Vectors
		pET Vectors
		pETBlue Vectors
		pETcoco Vectors
		pIEx Vectors
		pIEx/Bac Vectors
		pMLuc Vectors
		pT7Blue Vectors
		pTandem Vectors
		pTriEx Vectors
		SCREEN Vectors
		T7Select Vectors
		Discontinued Vectors

Duet Vectors

The Duet vectors are designed for the cloning and coexpression of two target ORFs. Every Duet vector contains two multiple cloning sites (MCS) each of which is preceded by a T7lac promoter and ribosome binding site (rbs). Multiple Duet vectors may be used together in compatible host strains to coexpress up to 8 target proteins (see detailed tables below).

pACYCDuet-1 carries the P15A replicon, lacI gene and chloramphenicol resistance gene.
pCDFDuet-1 carries the CloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance gene (aadA).
pCOLADuet-1 carries the ColA replicon, lacI gene and kanamycin resistance gene.
pETDuet-1 carries the pBR322-derived ColE1 replicon, lacI gene and ampicillin resistance gene.
pRSFDuet-1 carries the RSF1030 replicon, lacI gene and kanamycin resistance gene.

The vector sequences are provided in GenBank format, with significant features indicated. To convert the sequence into other formats, first select the sequence and copy (Command-C on Mac or Ctrl-C on Windows). Next, click here to open the NIH ReadSeq web page in a new window. Paste the sequence into the input field (Command-V on Mac or Ctrl-V on PC), choose your favorite format from the dropdown menu, and hit the "submit" button.

You can access sequences, maps, ordering information, newsletter articles and technical bulletins through the following links:

Vector	Sequence	Map	Ordering Information	Newsletter Articles	Technical Bulletins
pACYCDuet-1
pETDuet-1
pCDFDuet-1
pCOLADuet-1
pRSFDuet-1

Compatible Vector Combinations				Target Proteins (number)	Compatible Host Strain Group
Vector 1	Vector 2	Vector 3	Vector 4
pETDuet™-1 (Amp^R)	pACYCDuet-1 (Cam^R)	pRSFDuet-1 (Kan^R) or pCOLADuet-1 (Kan^R)	pCDFDuet-1 (Sm^R)	8	A
pETDuet-1 (Amp^R)	pACYCDuet-1 (Cam^R)	pCDFDuet-1 (Sm^R)	none	6	B
pETDuet-1 (Amp^R)	pACYCDuet-1 (Cam^R)	pRSFDuet-1 (Kan^R) or pCOLADuet-1 (Kan^R)	none	6	A
pETDuet-1 (Amp^R)	pRSFDuet-1 (Kan^R) or pCOLADuet-1 (Kan^R)	pCDFDuet-1 (Sm^R)	none	6	C
pRSFDuet-1 (Kan^R) or pCOLADuet-1 (Kan^R)	pACYCDuet-1 (Cam^R)	pCDFDuet-1 (Sm^R)	none	6	A
pETDuet-1 (Amp^R)	pRSFDuet-1 (Kan^R) or pCOLADuet-1 (Kan^R)	none	none	4	C
pACYCDuet-1 (Cam^R)	pETDuet-1 (Amp^R)	none	none	4	B
pETDuet-1 (Amp^R)	pCDFDuet-1 (Sm^R)	none	none	4	D
pRSFDuet-1 (Kan^R) or pCOLADuet-1 (Kan^R)	pCDFDuet-1 (Sm^R)	none	none	4	C
pACYCDuet-1 (Cam^R)	pRSFDuet-1 (Kan^R) or pCOLADuet-1 (Kan^R)	none	none	4	A
pACYCDuet-1 (Cam^R)	pCDFDuet-1 (Sm^R)	none	none	4	B
Resistance markers: Amp^R: ampicillin/carbenicillin, Kan^R: kanamycin, Cam^R: chloramphenicol, Sm^R: streptomycin/spectinomycin

Host Strain Groups
Group A B834(DE3) BL21(DE3) BLR(DE3) HMS174(DE3) Novablue(DE3) Origami 2(DE3)* Tuner™(DE3)	Group C B834(DE3) B834(DE3)pLysS BL21(DE3) BL21(DE3)pLysS BLR(DE3) BLR(DE3)pLysS HMS174(DE3) HMS174(DE3)pLysS NovaBlue(DE3) Origami 2(DE3)* Origami 2(DE3)pLysS* Rosetta(DE3) Rosetta(DE3)pLysS Rosetta 2(DE3) Rosetta 2(DE3)pLysS RosettaBlue(DE3) RosettaBlue(DE3)pLysS Rosetta-gami 2(DE3)* Rosetta-gami 2(DE3)pLysS* Tuner(DE3) Tuner(DE3)pLysS	Group D B834(DE3) B834(DE3)pLysS BL21(DE3) BL21(DE3)pLysS BLR(DE3) BLR(DE3)pLysS HMS174(DE3) HMS174(DE3)pLysS NovaBlue(DE3) Origami(DE3)* Origami(DE3)pLysS* Origami 2(DE3)* Origami 2(DE3)pLysS* Origami B(DE3) Origami B(DE3)pLysS Rosetta(DE3) Rosetta(DE3)pLysS Rosetta 2(DE3) Rosetta 2(DE3)pLysS RosettaBlue(DE3) RosettaBlue(DE3)pLysS Rosetta-gami(DE3)* Rosetta-gami(DE3)pLysS* Rosetta-gami 2(DE3)* Rosetta-gami 2(DE3)pLysS* Rosetta-gami B(DE3) Rosetta-gami B(DE3)pLysS Tuner(DE3) Tuner(DE3)pLysS
Group B B834(DE3) BL21(DE3) BLR(DE3) HMS174(DE3) NovaBlue(DE3) Origami(DE3)* Origami B(DE3) Origami 2(DE3)* Tuner(DE3)
These strains carry the rpsL* mutation that confers resistance to streptomycin; therefore, spectinomycin must be used for selection of pCDFDuet recombinants. Resistance markers: Amp^R, ampicillin/carbenicillin (50 �g/ml); Kan^R, kanamycin (30 �g/ml); Cam^R, chloramphenicol (34 �g/ml); Sm^R, streptomycin/spectinomycin (50 �g/ml). Note: the antibiotic concentrations used for four-way transformation plating and growth were reduced to 0.5 times the concentrations shown.