Overview
Affinity purification of GST fusion proteins
The GST•Bind and GST•Mag purification systems are based on the widely recognized affinity of glutathione-S-transferase (GST•Tag) fusion proteins for immobilized glutathione. Proteins are quickly and easily purified to near homogeneity in a single chromatographic step. Glutathione-resin based purifications require that the GST domain is soluble and properly folded. The gentle elution condition (10 mM reduced glutathione) avoids target protein denaturation. GST•Bind Resin utilizes an 11-atom spacer arm to covalently attach reduced glutathione via a sulfide linkage. The high degree of substitution of glutathione ensures a high binding capacity with yields of GST fusion proteins of 5 to 8 mg/ml settled resin. The resin can be re-used several times without loss of capacity.
GST•Mag Agarose Beads are available for rapid purification of multiple samples with minimum handling time. The 3-μm (average diameter) beads have binding capacity up to 2 mg/ml of settled resin as measured with GST protein and are easily collected with a magnet, which enables binding, wash, and elution procedures to be carried out in a single tube or well.
| GST•Bind Buffer Kit | | |
The GST•Bind Buffer Kit contains a set of pretested buffers for binding, washing and elution of GST•Tag fusion proteins from GST•Bind Resin or GST•Mag Agarose Beads. Sufficient components are provided to run a minimum of ten 2.5-ml GST•Bind columns.
Custom Configurations
GST•Bind Resin and GST•Mag Agarose Beads can be custom packaged in larger quantities to suit your needs. | | | | | | |
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