Gateway® Nova pET-DEST™ and pCOLA-DEST vectors are E. coli plasmid expression vectors which combine the advantages of the strong T7 promoter and expression control elements of the pET vectors with the convenience of Gateway Cloning Technology, which is sold and licensed by Invitrogen. Gateway technology allows the construction of many different expression clones for the same target by performing in vitro site-specific recombination between an entry clone (pENTR™) and various destination vectors (pDEST) to construct expression clones (pEXPR). A variety of Nova DEST vectors are offered to facilitate production of recombinant proteins with different fusion tags. Site-specific recombination between pENTR and Gateway Nova pDEST DNA occurs with att sequences (from "attachment site" for lambda prophage integration and excision with the bacterial host chromosome) and is catalyzed by Gateway® LR Clonase™ II Enzyme Mix (included in Gateway Nova DEST Expression Systems).
The vector sequences are provided in GenBank format, with significant features indicated. To convert the sequence into other formats, first select the sequence and copy (Command-C on Mac or Ctrl-C on Windows). Next, click here to open the NIH ReadSeq web page in a new window. Paste the sequence into the input field (Command-V on Mac or Ctrl-V on PC), choose your favorite format from the dropdown menu, and hit the "submit" button.You can access sequences, maps, ordering information, newsletter articles and technical bulletins through the following links: |
