CytoBuster™ Protein Extraction Reagent is a proprietary formulation of detergents optimized for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of CytoBuster enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw. CytoBuster has been specifically formulated for utilization in Western blotting, immunoprecipitation, and kinase/phosphatase assays. The reagent is compatible with protease inhibitors, kinase inhibitors and phosphatase inhibitors.



Analysis of S•Tag™ Fusion Proteins Extracted with CytoBuster™ Reagent
COS-1 cells were transfected with a pTriEx™ vector encoding the indicated S•Tag fusion proteins using GeneJuice™ Transfection Reagent. After 48 h the cells were treated with CytoBuster Protein Extraction Reagent and equal sample volumes analyzed by Coomassie stained SDS-PAGE (left panel) and Western blot (right panel). The S•Tag fusion proteins were detected on the Western blot using the S-protein AP Conjugate and NBT/BCIP AP substrates. The Perfect Protein™ Western Markers were detected simultaneously with the S-protein AP Conjugate.