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Detection Reagents for Strep•Tag® II  | | | | Back to Strep•Tag/Strep•Tactin Affinity Purification page | | | In addition to the powerful Strep•Tag® II expression vectors and Strep•Tactin® purification resin, detection tools for the Strep•Tag II coding sequence are available. The Strep•Tag II Monoclonal Antibody is a mouse monoclonal antibody (IgG1) that has high specificity and affinity for the 8 aa Strep•Tag II peptide. The purified antibody produces a strong signal for Western blots and dot blots. The Strep•Tag II Monoclonal Antibody can detect as little as 5 ng Strep•Tag II fusion protein and displays negligible cross-reactivity with bacterial, mammalian, and insect lysates. Also available is a Strep•Tag II Antibody HRP Conjugate, a peroxidase-conjugated Strep•Tag II Monoclonal Antibody, that is useful as a single detection reagent thus eliminating cross-reactivity associated with secondary reagents. The purified antibody conjugate produces a strong signal for Western blots and dot blots detecting as little as 7 ng Strep•Tag II fusion protein. |  | | | | To accurately determine the size of your protein, the Strep•Tag II Perfect Protein™ Markers are a mixture of Strep•Tag II fusion proteins ranging from 16 to 100 kDa. The markers provide precise size references for proteins on SDS-polyacrylamide gels after staining with Coomassie blue. Because each of the proteins contains the Strep•Tag II sequence, the markers can also be detected by the Strep•Tag II Monoclonal Antibody in Western blots. The Strep•Tag II Perfect Protein Markers include protein sizes of 16, 23.5, 30, 45, 60, and 100 kDa that resolve into sharp evenly stained bands. | | | Product | Size | Cat. No. | | Strep•Tag II Monoclonal Antibody | 100 mg | 71590-3 | | Strep•Tag II Antibody HRP Conjugate | 75 ml | 71591-3 | | Strep•Tag II Perfect Protein Markers | 100 lanes | 71614-3 |
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