InsectDirect™ System

InsectDirect Home   •  How does use of the       InsectDirect System benefit me?   •  What does the InsectDirect System offer?      •  Insect Cell Expression Vectors      •  Insect GeneJuice® Transfection Reagent      •  Insect RoboPop™         Ni-NTA His•Bind® Purification Kit         •  A brief protocol for the RoboPop kit         •  Comparison of traditional baculovirus             method and InsectDirect System method         •  Small- and large-scale Sf9 suspension             culture transfections

Insect GeneJuice® Transfection Reagent

Insect GeneJuice Transfection Reagent is a liposome-based transfection reagent optimized for maximal transfection of Spodoptera insect cells with minimal toxicity. It can be used for both transient and stable transfections in serum-containing or serum-free media. Insect GeneJuice is ideal for HT or large-scale protein expression when using the pIEx or pBiEx vectors for suspension culture transfection of Sf9 and other insect cells.

 

Sf9 cells transfected with pIEx-1/β-gal using Insect GeneJuice Transfection Reagent Transfected cells were stained for β-galactosidase activity using the X-Gal Solution–based BetaBlue™ Staining Kit.

Table 1: Comparison of transfection efficiency with different transfection reagents and cell lines, reported as raw relative light units (RLUs) from Rluc expression Transfection Agent/ Cell Line Combination Raw RLUs Fold Difference Insect GeneJuice®/Sf9 131,950 1.7 Other reagent/Sf9 75,726 Insect GeneJuice/High Five™ 31,897 2.7 Other reagent/High Five 11,952 Insect GeneJuice/Sf9 131,950 4.1 Insect GeneJuice/High Five 31,897 Insect GeneJuice/Sf9 131,950 11.0 Other reagent/High Five 11,952