InsectDirect™ System

InsectDirect Home   •  How does use of the       InsectDirect System benefit me?   •  What does the InsectDirect System offer?      •  Insect Cell Expression Vectors      •  Insect GeneJuice® Transfection Reagent      •  Insect RoboPop™         Ni-NTA His•Bind® Purification Kit         •  A brief protocol for the RoboPop kit         •  Comparison of traditional baculovirus             method and InsectDirect System method         •  Small- and large-scale Sf9 suspension             culture transfections

Insect RoboPop™ Ni-NTA His•Bind Purification Kit

• Insect RoboPop™ Ni-NTA His•Bind® Purification Kit is designed for filtration-based 96-well format purification of the His•Tag® fusion proteins directly from transfected insect cell cultures without centrifugation.The kit configuration allows for robotic processing of transfected 10-ml suspension cultures and purification of up to 400 µg His•Tag fusion protein per culture based on binding capacity of the resin.
  
  The kit includes:
  • Insect PopCulture® Reagent, a specially formulated detergent-based lysis reagent for protein extraction directly from total cultures
  • Benzonase® Nuclease, for viscosity reduction
  • Ni-NTA His•Bind Resin and buffers
  • 2-ml 96-well Filter Plate and Collection Plate with Sealer