| pBlueSTAR-1 is the plasmid that is formed by cre-loxP autosubcloning from the lambda parent vector, lambda BlueSTAR-1, and contains the 13,253 bp stuffer fragment in a plasmid backbone. The stuffer carries lacZ (including the lac promoter and operator) and inert EMC viral and lambda sequences. The lacZ gene will give a blue colony phenotype when plated in the presence of X-gal (strains carrying lacI may also require IPTG to give a blue color). To convert the sequence into other formats, first select the sequence and copy (Command-C on Mac or Ctrl-C on Windows). Next, click here to open the NIH ReadSeq web page in a new window. Paste the sequence into the input field (Command-V on Mac or Ctrl-V on PC), choose your favorite format from the dropdown menu, and hit the "submit" button. You can access sequences, maps, ordering information, newsletter articles and technical bulletins through the following links: |
