| The pETcoco™ Systems combine the advantages of T7 promoter-driven protein expression with the ability to control plasmid copy number. The pETcoco vectors are normally maintained at 1 copy per cell. In the single-copy state, pETcoco clones are extremely stable, especially if expression from the target gene is toxic to the host. Copy number can be amplified to 20-50 copies per cell by the additional of L-arabinose to the culture medium. The pETcoco vectors also possess the outstanding features of the pET vectors: in lDE3 lysogenic hosts, expression can be induced by as much as 2500-fold over background upon addition of IPTG. Cloning of target genes into pETcoco expression vectors and maintenance of stocks are best performed in a RecA-, EndA-, F- strain such as the NovaF- strain, which is supplied with the pETcoco Systems. The pETcoco vectors encode N-terminal His•Tag® and S•Tag™ fusion sequences, followed by protease cleavage site, multiple cloning site region, and optional C-terminal HSV•Tag® sequence. pETcoco-1 carries the chloramphenicol resistance gene, while pETcoco-2 carries the ampicillin resistance gene. The vector sequences are provided in GenBank format, with significant features indicated. To convert the sequence into other formats, first select the sequence and copy (Command-C on Mac or Ctrl-C on Windows). Next, click here to open the NIH ReadSeq web page in a new window. Paste the sequence into the input field (Command-V on Mac or Ctrl-V on PC), choose your favorite format from the dropdown menu, and hit the "submit" button. You can access sequences, maps, ordering information, newsletter articles and technical bulletins through the following links: |
