The pIEx™ vectors are designed to allow rapid, high-level protein expression in insect cells without the time-consuming process of creating a recombinant baculovirus. These vectors feature the hr5 enhancer and the IE1 (immediate early) promoter to direct expression in insect cells. This promoter/enhancer combination recruits endogenous insect cell transcription machinery, thereby avoiding baculovirus infection and the associated cytotoxic effects.
The pBiEx™ vectors are designed to allow rapid expression of target genes in both E. coli and insect cells, and to allow high-level, transient protein expression in insect cells without the time-consuming process of creating a recombinant baculovirus. These vectors feature the hr5 enhancer and the IE1 promoter to direct expression in insect cells. Expression in E. coli is regulated by the tightly controlled T7lac promoter. For target protein expression, pBiEx recombinant plasmids can be transferred into a (DE3) host that allows induction with IPTG. Expression can also be induced in hosts such as NovaBlue by infecting with lCE6, a phage that constitutively expresses T7 RNA polymerase.
The vector sequences are provided in GenBank format, with significant features indicated. To convert the sequence into other formats, first select the sequence and copy (Command-C on Mac or Ctrl-C on Windows). Next, click here to open the NIH ReadSeq web page in a new window. Paste the sequence into the input field (Command-V on Mac or Ctrl-V on PC), choose your favorite format from the dropdown menu, and hit the "submit" button.You can access sequences, maps, ordering information, newsletter articles and technical bulletins through the following links: |
