The pTandem™-1 Mammalian Dual Expression Vector is designed for coexpression of two genes in mammalian cells from a bicistronic RNA. This vector contains separate multiple cloning site located on both sides of an internal ribosome binding site (IRES). These features provide for convenient insertion of two open reading frames and efficient translation of both encoded proteins from a single bicistronic mRNA.
The pTK-neo vector can select stably transformed mammalian cell lines using G418. A minimal thymidine kinase (TK) promoter controls expression of the neomycin resistance gene. This promoter facilitates selection for stable integration of both the selection plasmid and a cotransfected expression plasmid into transcriptionally active sites in the genome. Try pTK-neo DNA as a cotransfection plasmid with pTandem™-1 Mammalian Dual Expression Vector, pTriEx™ vectors, pMLuc, or any mammalian expression vector.
The vector sequences are provided in GenBank format, with significant features indicated. To convert the sequence into other formats, first select the sequence and copy (Command-C on Mac or Ctrl-C on Windows). Next, click here to open the NIH ReadSeq web page in a new window. Paste the sequence into the input field (Command-V on Mac or Ctrl-V on PC), choose your favorite format from the dropdown menu, and hit the "submit" button.You can access sequences, maps, ordering information, newsletter articles and technical bulletins through the following links: |
