ProteoJuice™ Protein Transfection Reagent Efficient protein delivery into mammalian cells
ProteoJuice™ Protein Transfection Reagent efficiently delivers protein into mammalian cells. The most rapid method for studying the effects of a specific protein in a mammalian cell is to transfect the protein directly into the cell. Introduction of a protein into cells permits studies of protein-protein interactions, protein turnover, cell signaling pathways, apoptotic pathways, and transcription factor-mediated gene regulation. ProteoJuice™ Protein Transfection Reagent is an effective reagent for the transfection of intact functional protein into mammalian cells. 
Cells were transfected with 1.25 µg Cy™ 3-conjugated histone H2A by using ProteoJuice. Images depict fluorescent (panel A) and brightfield (panel B) views of the same cells. HepG2 cells were transfected with FITC-labeled estrogen receptor by using ProteoJuice. After incubation, washed cells were exposed to DAPI nuclear stain for 2 min, followed by four additional washes in PBS before microscopy. Panels A, B, and C: no estradiol treatment; panels D, E, and F: estradiol (Calbiochem, 17b-estradiol) treatment. Images depict brightfield (panels A and D), FITC fluorescence (panels B and E), and DAPI fluorescence (panels C and F) views, with the same cells in panels A, B and C and in panels D, E, and F. Arrows indicate estrogen receptor localization in nuclei. |
