Product Spotlight: Cell Invasion Assay (24-well) |
Cat. No.: QIA129
Format: 12 Tests
Kit Contents: 24-well plate containing 12 cell culture inserts coated with basement membrane extract, cell detachment solution, cell staining solution, cell staining diluent, and a directional insert.
Principle of the Assay: This Cell Invasion Assay utilizes an invasion chamber consisting of a 24-well tissue culture plate with 12 cell culture inserts and an 8 mm pore size polycarbonate membrane. The upper surface of the insert membrane is coated with a uniform layer of dried basement membrane matrix (BMM) solution. The layer of basement membrane solution forms an effective extracellular matrix protein barrier that prevents non-invasive cells from going through the 8 mm pores. Invasive cells, on the other hand, are able to degrade the matrix proteins that occlude the pores and allow them to pass through. Invaded cells cling to the bottom of the polycarbonate membrane that is tissue culture-treated to enhance cell attachment.
| 1. Cell suspension is placed in upper chamber and incubated for 24-72 hours at 37°C. 2. Invasive cells pass through the membrane and cling to the botton of the polycarbonate membrane that is tissue culture-treated to enhance cell attachment. Non-invading cells cannot pass through the membrane and stay in the upper chamber. |
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Labeling and dissociation of the invaded cells from the underside of the membrane are performed in one step. After transfer to a 96-well plate, samples can be measured in a microtiter plate fluorescence reader. No tedious manual sample processing, i.e. removing the biological matrix layer from the topside of the inserts with cotton tip applicators, or cell lysis step is required. Cells are quantified utilizing a highly sensitive fluorescent dye (Excitation: ~485 nm; Emission: ~520 nm).
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