New Cell Adhesion Assay Kits for Angiogenesis/Tumor Metastasis Research  |
The Innocyte™ Cell Adhesion Microplate Assays
are designed for the determination of the relative attachment of adherent cell lines to extracellular matrix proteins such as Human Fibronectin, Human Vitronectin, and Human Collagen IV. Cells are seeded onto a coated substrate, which is followed by the determination of relative cell attachment using a fluorescent dye.
• Detect cell adhesion activity with high sensitivity, without tedious pre-labeling of cells
• Study cell adhesion in a high-throughput format
• Obtain true quantitative cell adhesion data in a convenient fluorescence-based format
• Three different ECM-coated strips available
Background Information: The extracellular matrix (ECM) provides vital structure and organization to most organisms. It is an assemblage of collagens, proteoglycans, and glycoprotein such as fibronection and vitronectin. The ECM undergoes a constant remodeling that is most obvious during development, wound healing, and other repair processes (1-3). Fibronectin is a prominent constituent of the ECM around many cells, and fibronectin-rich matrices provide substrates for cell adhesion and migration during development, wound healing, and other processes, and affect many cellular functions, including proliferation, survival, and differentiation (4, 5). Vitronectin is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors (6). Collagen type IV is a prominent component of the basement membrane. Collagen IV can stimulate tumor cell proliferation, migration, and adhesion in a dose-dependent manner. Powerful cytokines such as TGF-b1 play an important role in stimulating the expression of collagen IV (7). Adhesive receptors on the cell surface such as integrins, selectins, cadherins, and ICAMs interact in a specific manner with different ECM proteins. Adhesion molecules reside in complex structures like focal adhesion adhesions and synaptic and adherens junctions, which also contain many specialized cytoskeletal proteins and signaling molecules (1).
References:
1. Horowitz, A.F., et al. 1996. Trends Cell Biol. 6, 460.
2. Hynes, R.O., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 2588.
3. Nelson, P.R., et al. 1997. Vasc. Surg. 26, 104.
4. Hynes, R.O., et al. 1990. Fibronectins, Springer-Verlag, New York.
5. Ohashi, T., et al. 1999. Proc. Natl. Acad. Sci USA 96, 2153.
6. Preissner, K.T., et al. 1990. J. Biol. Chem. 265, 18490.
7. Grande, J.P., et al. 1997. J. Lab. Clin. Med. 130, 476. |
Innocyte™ ECM Cell Adhesion Assay, Fibronectin
Cat. No. CBA011
A convenient assay for the determination of the relative attachment of adherent cell lines to fibronectin. Cells are seeded onto the fibronectin plates followed by determination of relative cell attachment using a fluorescent dye (Excitation max.: ~485 nm; emission max.: ~520 nm). Kit includes coated multi-well plate, fluorescent dye, D-PBS, and a directional insert. Suitable for use with adherent cultured cells.
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Innocyte™ ECM Cell Adhesion Assay, Vitronectin
Cat. No. CBA012
A convenient assay for the determination of the relative attachment of adherent cell lines to vitronectin. Cells are seeded onto the vitronectin plates followed by determination of relative cell attachment using a fluorescent dye (Excitation max.: ~485 nm; emission max.: ~520 nm). Kit includes coated multi-well plate, fluorescent dye, D-PBS, and a directional insert. Suitable for use with adherent cultured cells.
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Innocyte™ ECM Cell Adhesion Assay, Collagen Type IV
Cat. No. CBA013
A convenient assay for the determination of the relative attachment of adherent cell lines to collagen type IV. Cells are seeded onto the collagen type IV coated plates followed by determination of relative cell attachment using a fluorescent dye (Excitation max.: ~485 nm; emission max.: ~520 nm). Kit includes coated multi-well plate, fluorescent dye, D-PBS, and a directional insert. Suitable for use with adherent cultured cells.
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InnoCyte™ ECM Cell Adhesion Assay, Laminin/Basement Membrane Complex (BMC)
Cat. No. CBA025
The InnoCyte™ Cell Adhesion Assay, Laminin I/Basement Membrane Complex is designed for the determination of the relative attachment of adherent cell lines to laminin I and basement membrane protein complex, for evaluation of cell adhesion receptors, and for assessment of inhibitory or stimulatory substances for cell attachment. The kit is supplied with a 96-well strip plate coated with mouse laminin I and basement membrane complex (see plate format below). Cells are seeded in the coated wells and incubated at +37°C. Following incubation, the wells are washed briefly and attached cells are labeled the green fluorescent dye, calcein-AM. BSA-coated wells serve as a negative control and poly-L-lysine-coated wells serve as a positive control for general attachment. Relative cell attachment is assessed using a fluorescence plate reader. Includes laminin-coated 96-well plate strips, BMC-coated 96-well plate strips, calcein-AM solution, D-PBS, and user protocol.
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InnoCyte™ ECM Cell Adhesion Assay, Galectin-1/Galectin-3
Cat. No. CBA026
The InnoCyte™ Cell Adhesion Assay, Galectin-1/Galectin-3 is designed for the determination of the relative attachment of adherent cell lines to galectin-1 and galectin-3, for evaluation of cell adhesion receptors, and for assessment of inhibitory or stimulatory substances for cell attachment. The kit is supplied with a 96-well strip plate coated with galectin-1 and galectin-3. Cells are seeded in the coated wells and incubated at +37°C. Following incubation, the wells are washed briefly and attached cells are labeled the green fluorescent dye, calcein-AM. BSA-coated wells serve as a negative control and poly-L-lysine-coated wells serve as a positive control for general attachment. Relative cell attachment is assessed using a fluorescence plate reader. Includes galectin-1-coated 96-well plate strips, galectin-3-coated 96-well plate strips, calcein-AM solution, D-PBS, and user protocol.
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ECM Cell Adhesion Assay, Galectin-1/Galectin-3 to your shopping cart now:
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