		Technical Resources

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		Protease and Phosphatase Inhibitor Cocktails Resource

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Protease Inhibitor Cocktails		Phosphatase Inhibitor Cocktails
	Overview/Selection Guide	•	Overview/Selection Guide
•	Protease Inhibitor Cocktail Sets	•	Phosphatase Inhibitor Cocktail Sets
•	Properties of Selected Protease Inhibitors	•	Selected Protein Tyrosine Phosphatase Inhibitors
•	Frequently Asked Questions	•	Selected Serine/Threonine Phosphatase Inhibitors

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Protease Inhibitor Cocktails and Inhibitor Sets

Protease inhibitor cocktails and highly specific inhibitors to proteases are important tools for scientists engaged in a variety of proteomic studies including protein expression and characterization, biomarker discovery, mapping of post-translational modifications, protein expression profiling, and the quantitative measurement of proteins. The addition of a protease inhibitor cocktail or protease inhibitor ensures the integrity of proteins for downstream analysis and further characterization of samples.

During protein expression and isolation, endogenous proteases rapidly begin to degrade protein samples following cell lysis or tissue disaggregation. The resulting proteolysis can drastically reduce the quality and quantity of protein samples required for characterization and analysis. In order to overcome this problem, complex protein solutions are treated with a mixture or cocktail of protease inhibitors, which help preserve the activity and nature of proteins. By utilizing a specific combination of protease inhibitors, preparative protein samples may be protected from the most common proteases including serine proteases, metalloproteases, cysteine proteases, aminopeptidases, and aspartic proteases.

Using a combination of synthetic, recombinant, and naturally occurring protease inhibitors, we have developed a panel of 12 application specific protease inhibitor cocktails. These cocktails are formulated using reagents of the highest quality and biological activity. A convenient summary of the use of these cocktails is outlined in our convenient Protease Inhibitor Cocktail Selection Guide.

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Features:

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Convenient: Flexible protocol, Ready-To-Use formulations

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Consistent: High quality ensures reproducibility and excellent inhibition over a wide range of protease classes

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Flexible: A comprehensive selection of specific cocktail formulations designed to inhibit proteolytic activity from most tissue or cell type extracts, including mammalian, bacterial, yeast, fungal, and plant cells

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Application Specific Formulations:

–	Available without EDTA for purification schemes involving metal ion chelating chromatography or analysis using 2-D gel electrophoresis
–	Option of new protease inhibitor cocktail formulations using recombinant aprotinin for applications that require the use of animal-free reagents

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Selection Guide for the Use of Specialized Protease Inhibitor Cocktails

Product	Cat. No.	Components	Application
Protease Inhibitor Cocktail Set I	539131	AEBSF, HCl; Aprotinin, Bovine Lung; E-64 Protease Inhibitor; EDTA, Disodium, Leupeptin, Hemisulfate	General Use. Note: Reconstitute in 1 ml volume to obtain 100X stock solution. Dilute to 1X before use.
Protease Inhibitor Cocktail Set I, Animal-Free ^NEW!	535142	AEBSF, HCl; Aprotinin, Recombinant; E-64 Protease Inhibitor; EDTA, Disodium; Leupeptin Hemisulfate	General Use and for applications which require the use of animal-free reagents. Note: Reconstitute in 1 ml H₂O to obtain 100X stock solution. Dilute to 1X before use.
Protease Inhibitor Cocktail Set II	539132	AEBSF, HCl; Bestatin; E-64 Protease Inhibitor; EDTA, Disodium; Pepstatin A	Bacterial cell extracts (except those intended for metal chelation chromatography). Note: Reconstitute in 1 ml DMSO and 4 ml H₂O.
Protease Inhibitor Cocktail Set III, EDTA-Free	539134	AEBSF, HCl; Aprotinin, Bovine Lung; Bestatin; E-64 Protease Inhibitor; Leupeptin, Hemisulfate; Pepstatin A	Mammalian cells and tissue extracts purified using metal chelation chromatography; samples to be analyzed by 2-D gel electrophoresis. Note: Ready-to-use solution.
Protease Inhibitor Cocktail Set III, Animal-Free ^NEW!	535140	AEBSF, HCl; Aprotinin, Recombinant; Bestatin; E-64 Protease Inhibitor; Leupeptin, Hemisulfate; Pepstatin A	Mammalian cells and tissue extracts and for applications which require the use of animalfree reagents. Note: Ready-to-use solution.
Protease Inhibitor Cocktail Set IV	539136	AEBSF, HCl; E-64 Protease Inhibitor; Pepstatin A; o-Phenanthroline	Fungal and yeast cell extracts. Note: Ready-to-use solution.
Protease Inhibitor Cocktail Set V, EDTA Free	539137	AEBSF, HCl; Aprotinin , Bovine Lung; E-64 Protease Inhibitor; Leupeptin , Hemisulfate	Mammalian cells and tissue extracts purified using metal chelation chromatography; samples to be analyzed by 2-D gel electrophoresis. Note: Reconstitute in 1 ml H₂O to obtain 100X stock solution. Dilute to 1X before use.
Protease Inhibitor Cocktail Set V, Animal-Free ^NEW!	535141	AEBSF, HCl; Aprotinin , Recombinant; E-64 Protease Inhibitor; Leupeptin , Hemisulfate	Mammalian cells and tissue extracts and for applications which require the use of animalfree reagents. Note: Reconstitute in 1 ml H₂O to obtain 100X stock solution. Dilute to 1X before use.
Protease Inhibitor Cocktail Set VI	539133	AEBSF, HCl; Bestatin; E-64 Protease Inhibitor; Leupeptin, Hemisulfate; o-Phenanthroline; Pepstatin A	Plant cell extracts. Note: Ready-to-use solution.
Protease Inhibitor Cocktail Set VII	539138	AEBSF, HCl; Bestatin; E-64 Protease Inhibitor; Pepstatin A; Phosphoramidon, Disodium Salt	Proteins containing His•Tag� sequences. Note: Ready-to-use solution.
Protease Inhibitor Cocktail Set VIII	539129	ALLN; Cathepsin Inhibitor I; E-64 Protease Inhibitor	Broad range cysteine protease inhibition. Note: Ready-to-use solution
Serine Protease Inhibitor Cocktail Set I	565000	AEBSF, HCl; Aprotinin, Bovine Lung; Elastatinal, GGACK	Broad range serine protease inhibition. Note: Reconstitute in 1 ml H₂O to obtain 100X stock solution. Dilute to 1X before use.

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View PDF of Protease & Phosphatase Cocktail Selection Guide

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