		Technical Resources

		Technical Information

		Calbiochem Information

		Interactive Pathways

		Ubiquitin-Proteasome

Ubiquitin-Proteasome Interactive Pathway: Proteasome

�

Antibodies

Product

Application

Species Reactivity

Cat. No.

Anti-19S Regulator ATPase Subunit Rpt4 Mouse mAb (p42-23) IB, NOT IP human, mouse ST1059

Anti-19S Proteasome S5A-Subunit Rabbit pAb IB, IP human 539168

Anti-19S Proteasome S2-Subunit Rabbit pAb IB, IP human 539166

Anti-19S Proteasome, S6-Subunit Rabbit pAb IB, IP human 539169

Anti-20S Proteasome a1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231) ELISA, FS, IB, IC, IF, IH, NOT IP human, mouse, rabbit, rat, yeast ST1049

Anti-20S Proteasome a1-Subunit (249-263) Rabbit pAb IB canine, hamster, human, mouse, rat 539145

Anti-20S Proteasome a3-Subunit Mouse mAb (MCP257) IB, NOT IP, PS human, mouse, rabbit, rat ST1050

Anti-20S Proteasome a5-Subunit Mouse mAb (MCP196) IB, NOT IP, PS human, mouse, rabbit, rat ST1051

Anti-20S Proteasome a7-Subunit Mouse mAb (MCP72) IB, NOT IP, PS human, rabbit, rat, yeast ST1052

Anti-20S Proteasome b1-Subunit Mouse mAb (MCP421) IB, NOT IP human, rabbit ST1054

Anti-20S Proteasome b3-Subunit Mouse mAb (MCP102) IB, NOT IP human, mouse, rabbit, rat ST1055

Anti-20S Proteasome b4-Subunit (72-85) Rabbit pAb IB human, mouse, rat ST1056

Anti-20S Proteasome b5i-Subunit (23-223) Rabbit pAb IB, PS human, mouse, rabbit ST1057

Anti-20S Proteasome Core Subunits Rabbit pAb IB, IP, PS human, mouse, yeast ST1053

Anti-26S Proteasome S4-Subunit Rabbit pAb IB, IP human, yeast 539167

Anti-26S Proteasome, S7-Subunit Rabbit pAb IB, IP human 539171

Anti-Proteasome Activator 11S (PA28g) Subunit (1-14) Rabbit pAb IB human 539148

Anti-Proteasome Activator PA28a Subunit (5-22) Rabbit pAb IB canine, hamster, human, mouse, rat 539146

Anti-Proteasome Activator PA700 Subunit 10B (278-294) Rabbit pAb IB canine, hamster, human, mouse, rat 539147

Anti-19S Regulator ATPase Subunit Rpt1 Mouse mAb (MSS1-104) IB, NOT IP human, mouse ST1058

Anti-19S Regulator non-ATPase Subunit Rpn10 Mouse mAb (S5a-18) IB, IP human ST1060

Anti-Ubc9 Rabbit pAb IB, NOT FS, NOT PS human, mouse PC131

Anti-Ubiquitin (Ab-1) Mouse mAb (6C1) IB bovine, human CC37

Anti-Ubiquitin Rabbit pAb IB, IH, IP bovine, canine, chicken, Drosophila, E. coli, frog, guinea pig, hamster, human, monkey, mouse, rabbit, rat, sheep, yeast 662099

Anti-Ubiquitin-Activating Enzyme E1A Rabbit pAb IB, IH human 662102

Anti-Ubiquitin-Activating Enzyme E1A/E1B Rabbit pAb IB human 662106

�

Activators/Substrates

Product

Cat. No.

PA28 Activator, Rabbit 506280

Proteasome Substrate I, Fluorogenic 539140

Proteasome Substrate II, Fluorogenic 539141

Proteasome Substrate III, Fluorogenic 539142

Proteasome Substrate IV, Fluorogenic 539143

Proteasome Substrate VI, Fluorogenic 539149

Ubiquitin-AMC 662075

�

Assay Kits

Product

Cat. No.

Proteasome Isolation Kit, Human 539176

20S Proteasome Assay Kit, SDS-Activated 539158

26S Proteasome Degradation Kit 539159

�

Enzymes

Product

Cat. No.

Fraction II, Rabbit Reticulocytes 344260

�

Inhibitors

Product

Cat. No.

AdaAhx₃L₃VS 114802

AdaLys(Bio)Ahx₃L₃VS 114803

ALLM 208721

ALLN 208719

Epoxomicin, Synthetic 324800

InSolution™ Epoxomicin, Synthetic 324801

clasto-Lactacystin b-Lactone 426102

Lactacystin, Synthetic 426100

a-Methylomuralide 426104

MG-115 474780

MG-132 474790

InSolution™ MG-132 474791

NLVS 482240

NP-LLL-VS 492025

Proteasome Inhibitor I 539160

InSolution™ Proteasome Inhibitor I 539161

Proteasome Inhibitor II 539162

Proteasome Inhibitor III 539163

Proteasome Inhibitor IV 539175

Proteasome Inhibitor Set I 539164

Proteasome Inhibitor Set II 539165

Ro106-9920 557550

Ro106-9920, Control 557551

Tyropeptin A, Synthetic 657008

Ubiquitin Aldehyde 662056

UCH-L1 Inhibitor 662086

UCH-L3 Inhibitor 662089

�

Additional Information

�

Functional Class : proteasome endopeptidase complex


Name	proteasome endopeptidase complex
Description	multicatalytic endopeptidase complex
URN	urn:agi-enz:3.4.25.1
Total Controls	0
Total Nodes	0
Total Members	0
Notes	A 20-S protein composed of 28 subunits arranged in four rings of seven. The outer rings are composed of alpha subunits, but the beta subunits forming the inner rings are responsible for peptidase activity. In eukaryotic organisms there are up to seven different types of beta subunits, three of which may carry the N-terminal threonine residues that are the nucleophiles in catalysis, and show different specificities. The molecule is barrel-shaped, and the active sites are on the inner surfaces. Terminal apertures restrict access of substrates to the active sites. There is evidence that catalytic subunits are replaced by others under some conditions so as to alter the specificity of proteolysis, perhaps optimizing it for the formation of antigenic peptides. A complex of the 20-S proteasome endopeptidase complex with a 19-S regulatory unit is the 26-S proteasome that degrades ubiquitin-protein conjugates. Type example of peptidase family T1. Formerly EC 3.4.24.5, EC 3.4.22.21 and EC 3.4.99.46
Connectivity	0


Cell Localization	Cytoplasm


Alias	proteasome endopeptidase complex
	ingensin
	macropain
	multicatalytic endopeptidase complex
	prosome
	multicatalytic proteinase (complex)
	MCP
	proteasome
	large multicatalytic protease
	multicatalytic proteinase
	proteasome organelle
	alkaline protease
	26S protease
	tricorn proteinase
	tricorn protease


LocusLink ID	24967
	16912
	5698
	24968
	50601
	16913
	5696
	29666
	19175
	5694
	29668
	26440
	5682
	29669
	19166
	5683
	29670
	19167
	5684
	29671
	26441
	5685
	29672
	26442
	103084
	5686
	29673
	26443
	5687
	29675
	26445
	27983
	100341
	100397
	5690
	29676
	26446
	5691
	220568
	58854
	19172
	5692
	85492
	19177
	5695
	94198
	19170
	98079
	106483
	5689
	19171
	5699
	8138
	29425
	19173
	5693
	29674
	26444
	5688


EC Number	3.4.25.1


Class	Hydrolases
	Acting on peptide bonds (peptidases)
	Threonine endopeptidases


Genes	5682(PSMA1)
	5683(PSMA2)
	5684(PSMA3)
	5685(PSMA4)
	5686(PSMA5)
	5687(PSMA6)
	5688(PSMA7)
	5689(PSMB1)
	5690(PSMB2)
	5691(PSMB3)
	5692(PSMB4)
	5693(PSMB5)
	5694(PSMB6)
	5695(PSMB7)
	5696(PSMB8)
	5698(PSMB9)
	5699(PSMB10)


Disease	MIM: 176842 Proteasome (prosome, macropain) subunit, alpha type, 2
	MIM: 176843 Proteasome (prosome, macropain) subunit, alpha type, 3
	MIM: 176844 Proteasome component 5
	MIM: 600306 Proteasome subunit, beta type, 5
	MIM: 602017 Proteasome (prosome, macropain) subunit, beta type, 1
	MIM: 602175 Proteasome subunit, beta type, 2
	MIM: 602176 Proteasome subunit, beta type, 3
	MIM: 602177 Proteasome subunit, beta type, 4
	MIM: 602854 Proteasome subunit, alpha-type, 1
	MIM: 604030 Proteasome subunit, beta-type, 7
	MIM: 606607 Proteasome subunit, alpha-type, 7


PDB ID	1J2P
	1J2Q
	1Q5Q
	1Q5R

Reference

Seemuller, E., Lupas, A., Stock, D., Lowe, J., Huber, R. and Baumeister, W. Proteasome from Thermoplasma acidophilum: a threonine protease. Science 268 (1995) 579-582. 2 [PMID:8811196] Coux, O., Tanaka, K. and Goldberg, A.L. Structure and functions of the 20S and 26S proteasomes. Annu. Rev. Biochem. 65 (1996) 801-847. 3 [PMID:9087403] Groll, M., Ditzel, L., Lowe, J., Stock, D., Bochtler, M., Bartunik, H.D. and Huber, R. Structure of 20S proteasome from yeast at 2.4A resolution. Nature 386 (1997) 463-471. 4 [PMID:9748229] Dick, T.P., Nussbaum, A.K., Deeg, M., Heinemeyer, W., Groll, M., Schirle, M., Keilholz, W., Stevanovic, S., Wolf, D.H., Huber, R., Rammensee, H.G. and Schild, H. Contribution of proteasomal beta-subunits to the cleavage of peptide substrates analyzed with yeast mutants. J. Biol. Chem.386 (1998) 25637-25646.


DB Link	IUBMB Enzyme Nomenclature: 3.4.25.1
	ExPASy - ENZYME nomenclature database: 3.4.25.1
	ERGO genome analysis and discovery system: 3.4.25.1
	BRENDA, the Enzyme Database: 3.4.25.1
	CAS: 140879-24-9


Source	KEGG


CAS ID	140879-24-9

�

�

Change Country | Contact Us | Privacy Policy | Terms and Conditions

©2008 Calbiochem, Novabiochem, & Novagen

are brands of EMD Chemicals Inc., an Affiliate of Merck KGaA, Darmstadt, Germany.