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Diabetes and Obesity: Protein Tyrosine Phosphatases
 | | | | | | | Their Role in Diabetes and Obesity | | Insulin binding to the a-subunit of the insulin receptor stimulates the tyrosine kinase activity of the b-subunit, which results in phosphorylation of insulin-receptor substrates to propagate insulin signaling. Protein tyrosine phosphatases (PTP), particularly the protein tyrosine phosphatase-1B (PTP-1B), reduce the degree of phosphorylation of the insulin receptor, which diminishes insulin signaling and insulin sensitivity. A negative regulatory role for protein tyrosine phosphatases in insulin signaling and in the development of insulin resistance associated with Type 2 diabetes has been well established.PTP-1B is considered as a negative regulator of insulin signaling and several genetic studies have supported the association between PTP-1B and insulin resistance. Mice lacking PTP-1B exhibit enhanced insulin sensitivity and even when fed a high fat diet maintain their insulin sensitivity. They are resistant to diabetes and diet-induced obesity. Hence, PTP-1B is considered as an effective target for the treatment of both Type 2 diabetes and obesity. A variety of vanadium compounds have been known to mimic the effects of insulin by inhibition of tyrosine phosphatases. | | | | | | | Antibodies |
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| Anti-PTEN Mouse mAb (EMD-4B8) |
IB, NOT IP |
human |
AP1042 |
| Anti-PTEN Mouse mAb (EMD-15E10) |
ELISA, IP, NOT IB |
human |
AP1041 |
| PhosphoDetect™ Anti-PTEN (pSer380) Rabbit pAb |
IB, IC, IP, PS |
human, mouse, rat |
ST1072 |
| Anti-PTP1B (Ab-1) Mouse mAb (FG6-1G) |
IB, IF, IP |
avian, hamster, human, mouse (weakly), rat |
PH01 |
| Anti-PTP1B (Ab-2) Mouse mAb (AE4-2J) |
IB, IF, IP |
human |
PH02 |
| PhosphoDetect™ Anti-SHP-2 (pTyr542) Rabbit pAb |
IB, IP |
human, mouse, rat |
ST1082 |
| Anti-SHP-1 Mouse mAb (1SH01) |
IB |
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566806 |
| Anti-SHP-2 Rabbit pAb |
IB, IP, PS |
human, mouse, rat |
ST1083 |
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| | | | Assay Kits |
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| PTP Assay Kit, Fluorometric |
539742 |
| PTP1B Assay Kit, Colorimetric |
539736 |
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| PTP SHP-2, GST-Fusion, Human, Recombinant, E. coli |
565855 |
| PTPLAR, Human, Recombinant, E. coli |
539731 |
| PTP1B, Human, Recombinant, E. coli |
539735 |
| PTEN, His•Tag® and S•Tag™, Human Recombinant, S. frugiperda |
481409 |
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