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The InnoZyme™ TACE Activity Kit
 | | | | The InnoZyme™ TACE Activity Kit (Cat. No. CBA042) is designed to measure TACE activity in human cell lysates and other biological samples and for screening TACE inhibitors. The kit contains a 96-well plate pre-coated with a monoclonal antibody specific for human TACE that captures the enzyme. Unbound material is discarded, the plate is washed, and the activity of captured TACE is measured using an internally quenched fluorescent substrate, MCA-KPLGL-Dpa-AR-NH2. Cleavage of the scissile amide bond, G-L, releases the fluorophore from the quenching molecule, Dpa, resulting in an increase in fluorescence. Fluorescence of the cleaved product, MCA-KPLG, is measured at an exc. max. of ~324 nm and em. max. of ~400 nm. | | | • | Highly specific: based on immunocapture of human TACE | • | Highly sensitive and quantitative: assay range 5-100 ng/ml | • | Convenient: 96-well format and non-radioactive detection | • | Versatile: appropriate for measuring active TACE and high-throughput screening of TACE inhibitors |
| | |  | Figure 1. Activity of recombinant human TACE |
| | |  | Figure 2. Activity of TACE/ADAM17 in biological samples
Human colorectal adenocarcinoma cells, DLD1 and HT-29 and human glioblastoma cells, T98G, were grown in DMEM medium supplemented with 10% FCS and harvested at 80-90% confluency. Cell lysates were prepared using CytoBuster™ Protein Extraction Reagent. |
| | | | |  Related Products | | | CytoBuster Protein Extraction Reagent | | | TACE, His•TagŪ, Recombinant Protein | | | TACE Substrate II, Fluorogenic | | | TACE Substrate III, Fluorogenic | | | TACE Substrate IV, Fluorogenic | | | TAPI-0 | | | TAPI-1 | | | TAPI-2 | |
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