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NEW! K-LISA™ Aurora Kinase Activity Kit
 | | | | Principle of Assay | Assay Characteristics and Examples | Related Products | | | Aurora A and B are evolutionarily conserved serine/threonine kinases that play a critical role in chromosome segregation and cell division. Aurora kinases have been implicated in the centrosome cycle, spindle assembly, chromatin condensation, and the spindle checkpoint. Aurora A is upregulated during mitosis and localizes to spindle poles and spindle microtubules during early mitosis. It is targeted to spindles by the spindle component, TPX2, and is degraded during the onset of anaphase as a result of ubiquitin dependent proteolysis. Aurora A is activated by phosphorylation and, in turn, phosphorylates substrates including Histone H3, CDC25B, and p53. Aurora B kinase is a critical mitotic regulator that localizes to the inner centromere region from prophase to anaphase. It plays a role in regulating microtubule dynamics, chromosome congression, and the spindle checkpoint. Aurora B, like Aurora A, is activated by phosphorylation and subsequently phosphorylates substrates such as Histone H3 and vimentin. Phosphorylation of Histone H3 on Ser10 during mitosis by Aurora B is associated with chromatin condensation and histone acetylation.
Aurora kinases have been linked to cancer progression and their chromosomal loci are frequently altered in human tumors. Elevated expression of Aurora is found in breast, bladder, colon, ovarian, and pancreatic cancers. Ectopic expression leads to rodent cell transformation and overexpression in human cell lines leads to tetraploidy. Genetic studies identified Aurora A as a cancer susceptibility gene in both human and mouse. Inhibition of Aurora kinases represents a potential rationale for cancer drug development. | | | | Principle of Assay | | The K-LISA™ Aurora Activity Kit (Cat. No. CBA051) is suitable for measuring the kinase activity of purified or partially purified preparations of Aurora A and Aurora B. The assay is also useful for in vitro Aurora A and Aurora B inhibitor screening and for assessing the regulation of Aurora A and Aurora B in cell signaling. Recommended sample types include Aurora A or Aurora B that has been immunoprecipitated from crude cell lysates or purified Aurora A or Aurora B proteins. | | | | The K-LISA™ Aurora Activity Kit is: | • | Versatile: can detect kinase activity or be used for inhibitor screening | • | Convenient: 96-well ELISA-based format, non-radioactive detection | • | Easy Detection: Colorimetric, using HRP-Antibody Conjugate and TMB Substrate |
| | | | Assay Characteristics and Examples | | | | | | | | | | | | | | | | | | Related Products | | | Antibodies
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| Anti-ATM (Ab-1) (13-24) Rabbit pAb |
IB |
human, mouse |
PC85 |
| Anti-ATM (Ab-2) Mouse mAb (6B7) |
IP, NOT IB |
human |
OP90 |
| Anti-ATM (Ab-3) (819-844) Rabbit pAb |
IB, IP |
human |
PC116 |
| Anti-ATM (Ab-3) Rabbit pAb |
IB, IP |
human |
PC116T |
| Anti-ATR (Ab-2) (2122-2644) Rabbit pAb |
IB |
hamster, human, mouse |
PC538 |
| Anti-ATR (Ab-2) Rabbit pAb |
IB |
hamster, human, mouse |
PC538T |
| Anti-Aurora A Mouse mAb (35C1) |
IB |
human, mouse |
PK11 |
| Anti-Chk1 (1-474) Sheep pAb |
IB |
human |
PC423 |
| PhosphoDetect™ Anti-Chk1 (pSer317) Rabbit pAb |
IB, IC, PS |
human, monkey, mouse, rat |
DR1025 |
| Anti-Chk2 (Ab-2) Mouse mAb (2CHK01) |
IB, IF, IP |
human |
CC44 |
| PhosphoDetect™ Anti-Chk2 (pThr68) Rabbit pAb |
FC, IB, IC, IH, IP |
human, monkey |
DR1026 |
| Anti-Chk2 Rabbit pAb |
IB, IP |
human |
DR1036 |
| Anti-PCNA (112-121) (Ab-1) Mouse mAb (PC10) |
IB, IF, NOT IP, PS |
human, mouse, rat, yeast |
NA03T |
| Anti-PCNA (247-261) (Ab-5) Rabbit pAb |
IP |
human |
PC474 |
| Anti-PCNA (Ab-1) Mouse mAb (PC10) |
IB, IF, NOT IP, PS |
human, mouse, rat, yeast |
NA03 |
| Anti-PCNA (Ab-1) Mouse mAb (PC10) Fluorescein Conjugate |
FC, IF |
human, mouse, rat, yeast |
NA03F |
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| | | Kits
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| K-LISA™ Checkpoint Activity Kit |
96-well plate |
96 Tests |
25 pg (0.018 mU) purified Chk1 |
25-725 µU (Chk1) and 260 µU - 26 mU (Chk2) |
3 h |
Cell lysates, tissue extracts, purified enzymes |
CBA020 |
| PCNA ELISA Kit |
96-well strip plate |
96 Tests |
0.5 U/ml of PCNA |
1.875 - 30 U/ml |
3.5 h |
Cell lysates, tissue culture media , serum, or plasma |
QIA59 |
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| | | Inhibitors
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| Chk1, His•Tag®, Human, Recombinant, S. frugiperda |
220479 |
| Chk2, His•Tag®, Human, Recombinant, S. frugiperda |
220487 |
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| | | | Related Pathway: Checkpoint Signaling and DNA Repair |  | | | | | | | |
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