K-LISA™ Checkpoint Activity Kit (Cat. No. CBA020) and Related Products
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| Mammalian cells protect their genomic DNA against environmental and metabolic insults by a series of surveillence pathways known as cell-cycle checkpoints. These checkpoints ensure high fidelity maintenance of genomic DNA and prevent inappropriate inheritance of damaged DNA. The checkpoint programs arrest the cell cycle allowing for either DNA repair or completion of DNA synthesis. Two checkpoint serine/threonine kinases, Chk1 and Chk2, whose activity is required for the normal G2 checkpoint response to a variety of cellular stresses, play an integral role in these processes. The K-LISA™ Checkpoint Activity Kit is a rapid, sensitive ELISA-based activity assay suitable for measuring kinase activity of purified or partially purified Chk1 and Chk2 preparations, in vitro Chk1 and Chk2 inhibitor screening, and for assessing the regulation of Chk1 and Chk2 in cell signaling which may aid in discovery of potential new targets of cancer therapies. The assay utilizes an N-terminal biotinylated peptide substrate (KKKVSRSGLYRSPSMPENLNRPR) that is phosphorylated on the third serine by Chk1 and Chk2. The phosphorylated substrate is detected with a phosphoserine detection antibody, followed by an anti-IgG HRP conjugate and color development with TMB substrate. |
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Features of K-LISA Checkpoint Activity Assay Kit (Cat. No. CBA020): - Highly versatile: can detect kinase activity in purified or partially purified Chk1 and Chk2 preparations.
- Highly sensitive: can detect kinase activity at picogram (Chk1) and nanogram levels (Chk2).
- Highly convenient: 96-well format, non-radioactive detection.
- Highly useful: enables high throughput screening of kinase inhibitors.
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| Application Examples: |
 | Activity of purified Chk1: The activity of HisTag®, Human, Recombinant Chk1 (17 - 2200 pg) was determined using the K-LISA™ Checkpoint Activity Kit. Assay range: 34 pg - 1100 pg (740 Units/mg). |
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 | Activity of purified Chk2: The activity of Human, Recombinant Chk2 (0.06 - 60 ng) was determined using protocol A in the Detailed Protocol section. Assay range: 200 pg – 20 ng (1283 Units/mg). |
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| Additional Related Products: |
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| New Antibodies | | PhosphoDetect™ Anti-ATM (pSer1981) Mouse mAb (10H11-E12) | | | Anti-BLM Rabbit pAb | | | PhosphoDetect™ Anti-CHK2 (pThr68) Rabbit pAb | | | PhosphoDetect™ Anti-CHK1 (pSer317) Rabbit pAb | | | PhosphoDetect™ Anti-H2AX (pSer139) Rabbit pAb | | | Anti-H2AX, Rabbit pAb | | | Anti-MCM10 Rabbit pAb | | | Anti-NBS1 Rabbit pAb | | | Anti-NFBD1/MDC1, Rabbit pAb | | | Anti-p53 Binding Protein 1 (BP13) Mouse mAb (BP13) | | | Anti-Pso4, Rabbit pAb | | | Anti-SMC6 Rabbit pAb | | | Anti-WRN Rabbit pAb | | | View all DNA Repair Antibodies |
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| Proteins | | Chk1, His•Tag®, Human, Recombinant | |
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| Related Pathways: |
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| Mitochondrial Apoptosis Pathway |
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| Other Resources: |
| K-LISA™ Kits |
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