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ProteoExtract ® Abundant Protein Removal Kits  |
Enhancing resolution of low abundance proteins...
A major challenge for disease marker identification in human serum or plasma is the extraordinary range of protein abundance present in the sample. Proteins in plasma differ in concentration by a factor of one billion. Serum albumin can constitute 55% of total serum proteins and IgG can be as much as 25%. The presence of these proteins makes the resolution of lower-abundance proteins difficult. The new ProteoExtract® Abundant Protein Removal Kits provide highly specific and efficient depletion of either albumin alone or albumin and IgG from serum, plasma, or cerebrospinal fluid (CSF). Removal of serum albumin and IgG eliminates approximately 75% of the total protein present in serum. A sample enriched three to four times can be loaded on 2D gels or liquid chromatography columns. Sample complexity is significantly reduced, thereby improving the detection and analysis of low-abundance proteins and peptides in biomarker discovery, toxicological studies for new drugs, protein profiling using SELDI analysis, and other applications.
| | |  ProteoExtract® Albumin Removal Kit ( Cat. No. 122640 )
The ProteoExtract Albumin Removal Kit is based on a new affinity resin which is highly specific for albumin. Depletion of albumin from a typical human serum sample is consistently higher than 80% without binding significant amounts of other serum proteins. The remarkable selectivity provided by the resin and the optimized design of the columns result in less than 10% background binding of other serum proteins. Each kit contains 12 disposable columns pre-packed with 300 µl albumin-binding resin and a binding buffer for optimal resin performance. Resin binding capacity is 2 mg albumin per column and sample volumes of up to 60 µl can be processed without any loss of selectivity. The gravity-flow column format minimizes hands-on time making it highly suitable for processing multiple samples in parallel. The ProteoExtract Albumin Removal Kit depletion procedure is convenient and straightforward: equilibrate, load the diluted sample, allow to pass by gravity-flow, wash the column, combine collected flow-through and wash, and concentrate the proteins. Depleted samples are compatible with 1DGE, 2DGE, LC/MS, and MALDI-TOF MS. Although ProteoExtract Albumin Removal Kit has been optimized to bind human serum albumin, it is also compatible with rabbit, rat, and mouse samples.
 Features:
- Efficient removal of albumin with albumin-specific resin (not Cibacron based)
- Highly specific, exhibiting little non-specific binding
- Increased loading of enriched sample on 2DGE or LC
- Fast, 20 - 30 minute protocols
- Easy, column-based procedure to process multiple samples in parallel |
The ProteoExtract® Albumin Removal Kit binds albumin with high capacity and very low background binding to other serum proteins: | | | The ProteoExtract Albumin Removal Kit allows for the visualization of many proteins previously non-detected: | | | Unprocessed Human Serum | | Albumin Depleted Human Plasma | Fig. 3A |  | Fig. 3B |  |
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 Add ProteoExtract® Albumin Removal Kit (Cat. No. 122640) to your shopping cart now:
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| * NOTE: In Stock status is based on item availability worldwide. |
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| | | ProteoExtract® Albumin/IgG Removal Kit (Cat. No. 122642 )
The ProteoExtract Albumin/ IgG Removal Kit uses a combination of the albumin-specific resin and a unique immobilized protein A polymeric resin. Depletion of albumin and IgG from typical human serum samples is consistently higher than 80% without binding significant amounts of other serum proteins. The excellent selectivity provided by the resins and the optimized design of the columns result in less than 10% background binding of other serum proteins. Sample volumes up to 60 µl can be processed without any loss of selectivity. The ProteoExtract Albumin/IgG Protein Removal Kit has been optimized to bind human serum albumin and IgG, but will also deplete rabbit, rat, mouse, or pig samples effectively. Each kit contains 12 disposable columns pre-packed with 450 µl of the resin mix and an optimized binding buffer that promotes selective binding of albumin and IgG. Binding capacity of each column is 0.7 mg IgG and 2 mg albumin. The gravity-flow column format minimizes hands-on time, allowing the parallel processing of multiple samples. The kit procedure is similar to the ProteoExtract Albumin Removal Kit procedure. Depleted samples are compatible with downstream proteomics methods such as 1D and 2D gel electrophoresis, LC/MS, and MALDI-TOF MS.
| | Features:
- Efficient removal of albumin and IgG from serum samples
- Highly specific, exhibiting very little non-specific binding
- Compatible with human, rabbit, rat, mouse, and pig serum samples
- Increased loading of enriched sample on 2D gel electrophoresis or LC
- Fast, 20–30 minute protocols
- Easy, column-based procedure to process multiple samples in parallel |
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Add ProteoExtract® Albumin/IgG Removal Kit (Cat. No. 122642) to your shopping cart now:
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| * NOTE: In Stock status is based on item availability worldwide. |
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| | | | ProteoExtract® Abundant Protein Removal Kits Maxi | | Proteins in plasma differ in concentration by a factor of one billion. Serum albumin can constitute 55% of total serum proteins and IgG 10-25%. The presence of these proteins can obscure others and make the resolution of lower abundant proteins difficult. The new ProteoExtract® Abundant Protein Removal Kits Maxi provide a convenient, reproducible and highly specific method for albumin and IgG depletion from up to 180 µl of plasma, serum, or cerebrospinal fluid. These unique columns are designed to be used manually as syringe tip filters or connected to any standard HPLC/FPLC instrument. | | | Features:
- Efficient removal of albumin with albumin-specific resin (not Cibracon based)
- Highly specific, exhibiting little non-specific binding
- Compatible with samples from various species (e.g., human, mouse, rabbit, rat, etc.)
- Fast, 20 minute manual protocol
- Easy, column-based format compatible with manual syringes and LC-instrumentation
- Reusable, 10 reactions/column | | |
Add ProteoExtract® Albumin Removal Kit Maxi (Cat. No. 122641) to your shopping cart now:
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| * NOTE: In Stock status is based on item availability worldwide. |
| ** Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Please contact your local sales office for more information regarding these charges. |
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| | Add ProteoExtract® Albumin / IgG Removal Kit Maxi (Cat. No. 122643) to your shopping cart now:
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| * NOTE: In Stock status is based on item availability worldwide. |
| ** Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Please contact your local sales office for more information regarding these charges. |
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