Efficient and standardized extraction of cellular proteins into four distinct partial proteomes...
 Overview:
ProteoExtract® Partial Proteome Extraction Kit (P-PEK) provides efficient and standardized extraction of bacterial, yeast, and mammalian cellular proteins into four distinct partial proteomes. Analysis of the partial proteome fractions enriched in subsets of cellular proteins allows for the detection of low-abundance proteins by 2D gel electrophoresis and Western blot analysis.
P-PEK contains uniquely balanced combinations of chaotropes and detergents to solubilize and enrich a different subset of cellular proteins in each of four fractions. The most soluble proteins are released by mechanical disruption in extraction reagent 1, while extraction reagent 2 releases proteins of intermediate solubility. Reagent 3 provides efficient extraction of membrane proteins. SDS buffer solubilizes proteins still insoluble in reagent 3.
Effective removal of nucleic acids with Benzonase® Nuclease eases sample handling and highly improves resolution on 2D gels. P-PEK protocols generally work without a sonication step, thereby avoiding protein modifications due to temperature effects. P-PEK–extracted proteins are ready for immediate use in 2D gel electrophoresis applications.
Features:
- Specifically developed and tested protocols for different sample types
- Protein fractions enriched in subsets of cellular proteins to visualize low-abundance proteins
- Ready-to-use proteins for 1D and 2D gel electrophoresis
- Excellent spot resolution through Benzonase Nuclease addition
- Includes protease inhibitors to preserve protein profiles
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Add ProteoExtract® Partial Mammalian Proteome Extraction Kit (Cat. No. 