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ProteoExtract ® All-in-One Trypsin Digestion Kit & Tryptic Cleavage Modification Kit  | All-in-One Trypsin Digestion Kit | Tryptic Cleavage Modification Kit ProteoExtract® All-in-One Trypsin Digestion Kit Overview: The ProteoExtract ® All-in-One Trypsin Digestion Kit contains a set of optimized reagents that allows tryptic digests of various protein sample types: spots or bands excised from polyacrylamide gels, protein solutions, or cell and tissue extracts. Each protocol is optimized using affinity-purified trypsin, which guarantees efficient digestion of protein samples regardless of their origin. High digestion efficiency results in a high yield of tryptic peptides—even with hard-to-digest proteins—leading to improved LC-MS peak patterns and sequence coverage after MS analysis. This allows a comprehensive analysis of post-translational modifications such as phosphorylation site identification. Features:
- Compatible with a variety of protein sample types - Fast procedure, complete digestion in 3 hours - 20–30% increase in sequence coverage Application Example :
The ProteoExtract Digestion Kit generates greater MS sequence coverage compared to a competitor product. As given in Table 1 certain amounts of proteins were separated on a 12% Tris-glycine SDS-PAGE gel. The gel was stained with colloidal Coomassie™, the bands were excised, destained and tryptically digested with the ProteoExtract® Trypsin Digestion Kit (2 hrs) or with a competitor product (4 hrs) according to the manufacturers’ protocols. The tryptic peptides were then analyzed by LC-MS/MS with subsequent database search using the Mascot algorithm and the SWISS-PROT/TrEMBL database. | | | Table 1: The ProteoExtract® Trypsin Digestion Kit leads to the indicated better percentage in
MS/MS sequence coverage for these three proteins compared to the competitor product. | | Proteins | Origin | kDa | Conc. pmol. | % Increase MS/MS sequence coverage | | Carbonic Anhydrase | Bovine Erythrocytes | 30 | 16,6 | 29,31 | | Ovalbumin | Chicken Egg | 43 | 11,6 | 25,49 | | BSA | Bovine Serum | 67 | 7,4 | 28,62 |
| | | | | | | | |  For additional product information, insert, MSDS, and C of A, please visit the product detail page for the ProteoExtract® All-in-One Trypsin Digestion Kit, Cat. No. 650212 | | |
 Add ProteoExtract® All-in-One Trypsin Digestion Kit (Cat. No. 650212) to your shopping cart now:
| | | ProteoExtract® Tryptic Cleavage Modification Kit Overview:
Proteolytic digestion of proteins is an important step for subsequent protein characterization and identification by mass-spectrometry (MS). Traditionally trypsin is the protease of choice and is described for its efficiency, substrate specificity and reliability. To facilitate a detailed protein sequence characterization, digestion usually is repeated with different enzymes like Arg-C, Lys-C, Glu-C etc. However, the efficiency, specificity and reliability of these enzymes is by far inferior to that of trypsin. The ProteoExtract Tryptic Cleavage Modification kit is designed to utilize trypsin with its advantages in an optimized protocol applying the ProteoExtract® All-in-One Trypsin Digestion kit and additionally to control its cleavage-site usage by innovative and specific chemical modification steps resulting in either trypsin, Arg-C- or Lys-C-like digested peptide fragments for MS/MS analysis.
Features:
- Innovative and specific chemical modification to control the trypsin cleavage site during in-gel digestion
- Modification efficiency typically of >90% depending on protein structure and composition
- Improved LC-MS peak patterns and sequence coverage
- Amenable for MS/MS and ESI-LC-MS/MS techniques | | |
Add ProteoExtract® Tryptic Cleavage Modification Kit (Cat. No. 539182) to your shopping cart now:
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