His•Bind and His•Mag Matrix Selection Guide
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| • His•Bind and His•Mag Matrix Selection Guide | |
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| Product | Form | Capacity | Features | Applications | Size | Cat. No. |
Ni-NTA His•Bind
Resin | Ni-charged
NTA
agarose | 5–10 mg/ml | Minimal Ni2+leaching Compatible with 20 mM b-ME and 1mM THP
Compatible with Ni-NTA Buffer Kit | Small to medium scale
Gravity flow column
Recommended for eukaryotic extracts | 10 ml
25 ml
100ml | 70666-3
70666-4
70666-5 |
Ni-NTA His•Bind
Superflow | Ni-charged
NTA
Superflow
agarose | 5–10 mg/ml |
Minimal Ni2+leaching
Compatible with 20 mM b-ME and 1mM THP
Compatible with Ni-NTA Buffer Kit
High flow rates and pressures
| Small to production scale
FPLC or gravity flow column
Recommended for eukaryotic extracts | 10 ml
25 ml
100ml | 70691-3
70691-4
70691-5 |
His•Bind
Resin | Uncharged
IDA agarose | 8 mg/ml |
Reusable many times
Compatible with His•Bind Buffer Kit
Compatible with 1 mM THP
| Small to medium scale
Gravity flow column or batch mode | 10 ml
25 ml
100ml | 69670-3
69670-4
69670-5 |
His•Bind Column | Ni-charged
IDA agarose,
pre-packed column | 10 mg |
Pre-packed column
Compatible with His•Bind Quick Buffer Kit
Compatible with 1 mM THP
| Convenient purification
Gravity flow column | pkg/5
pkg/25 | 70971-3
70971-4 |
His•Bind Fractogel (M) | Uncharged
Tentacle IDA
methacrylate | > 10 mg/ml |
40–90 μM particle size
High flow rates and pressures
| Small to production scale
FPLC or gravity flow column | 25 ml | 70693-3 |
His•Bind
Quick
300 Cartridge | Ni-charged
IDA cellulose
packed cartridge | 0.5 mg |
Luer fitting on each end
Compatible with His•Bind
Quick Buffer Kit
| Syringe-driven processing
Vacuum manifold processing
Rapid purification | pkg/10
pkg/50 | 70155-3
70155-4 |
His•Bind
Quick
900 Cartridge | Ni-charged
IDA cellulose
packed cartridge | 2 mg |
Luer fitting on each end
Compatible with His•Bind
Quick Buffer Kit
| Syringe-driven processing
Vacuum manifold processing
Rapid purification | pkg/10
pkg/50 | 70156-3
70156-4 |
His•Bind
Quick Column | Ni-charged
IDA cellulose
packed cartridge | 5 mg |
Luer fitting on one end
Compatible with His•Bind
Quick Buffer Kit
| Vacuum manifold processing
Rapid purification of multiple
samples | pkg/12
pkg/60 | 70159-3
70159-4 |
His•Mag
Agarose
Beads | Ni-charged
IDA magnetic
agarose | 5 mg/ml | 3 μm magnetic agarose beads |
Rapid small scale purification
Magnetic separation
HT-compatible
|
2x1 ml
10x1 ml
| 71002-3
71002-4 |
His•Bind Resin, Pre-charged | Ni-charged
IDA agarose | 8mg/ml |
Reusable many times
Compatible with His•Bind Buffer Kits
Compatible with 1mM THP
| Small to medium scale
Gravity flow column or batch mode | 10 ml
25 ml
100ml | 71035-3
71035-4
71035-5 |
| Note: as with any affinity matrix, the cleanest separations are achieved when a His•Bind resin is used near its binding capacity |
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