Liquid. In 150 mM NaCl, 50 mM Tris-HCl, pH 7.5. AVOID FREEZE/THAW CYCLES. Recombinant, human 14-3-3b, fused to GST at the N-terminus with a thrombin cleavage site and expressed in E. coli. Useful for protein-protein interaction assays and gel overlays. The 14-3-3 protein family consists of a group of highly conserved proteins that may regulate the function of signal transduction complexes by modulating their enzymatic activity, conformation, stability, and/or intracellular localization. 14-3-3b plays an important role in the proliferation and oncogenic transformation of NIH3T3 cells through enhancement of Raf-1 activation and resultant augmentation of signaling in the MAPK cascade. Purity: ≥90% by SDS-PAGE.

Ref.: Ellis, J.J., et al. 2002. Mol. Cell. Biol. 22, 6809. Yaffe, M.B., et al. 2002. FEBS Lett. 513, 53. Grozinger, C.M. and Shreiber, S.L. 2000. Proc. Natl. Acad. Sci. USA 97, 7835. Takihara, Y., et al. 2000. Carcinogenesis 21, 2073. Furlantetto, R.W., et al. 1997. Biochem. J. 327, 765.