Lyophilized. Lyophilized from sterile filtered 35% acetonitrile, 0.1% TFA, 50 µg BSA/µg Activin A. Recombinant, human activin A (disulfide-linked homodimer of two 116-amino acid residue bA subunits) expressed in CHO cells. Mature bA-monomer lacks a potential N-linked glycosylation site and has a calculated molecular weight of 13 kDa. A member of the TGF-b superfamily that is involved in the negative regulation of B lineage lymphocytes. Shown to play an important role in the regulation of hormonogenesis, cellular homeostasis, and differentiation programs. Reported to inhibit endogenous production of IL-6. Biological activity: ED50 = 0.5-2.0 ng/ml as measured by its ability to induce hemoglobin expression in K562 cells. Purity: ≥95% by SDS-PAGE. Contaminants: Endotoxin: ≤100 pg/µg Activin A. Ref.: Zipori, D., et al. 2001. J. Leukoc. Biol. 69, 867. Hedger, M.P., et al. 2000. Cytokine 12, 595. Woodruff, T.K. 1998. Biochem. Pharmacol. 55, 953. Ying, S.Y., et al. 1997. Proc. Soc. Exp. Biol. Med. 214, 114. Schwall, R.H., and Lei, C. 1991. Methods Enzymol. 198, 340. Mason, A., et al. 1986. Biochem. Biophys. Res. Commun. 135, 957. |
