Format: 96-well format

Form: 100 Tests

Detection Method: Colorimetric

Sensitivity: 390 fmol/ml (non-acetylated), 37 fmol/ml (acetylated)

Assay Range: 0.78 - 200 pmol/ml (non-acetylated), 0.078-20 pmol/ml (acetylated)

Assay Time: 4.5 h

Sample Type: Serum, cell, and tissue samples

Kit Contents: One Antibody Coated 96-Well Plate, cAMP Direct Conjugate, Anti-cAMP, cAMP Standard, 0.1 N HCl, Neutralizing Reagent, Wash Buffer, Color Substrate, Stop Solution, Triethylamine, Acetic Anhydride, a Plate Sealer, and a user protocol.

Comments: A non-radioactive competitive immunoassay kit for the rapid quantitative measurement of cAMP. No neutralization, extraction, drying, or reconstitution of samples is required. Samples are treated with 0.1N HCl to lyse the cells, stop endogenous phosphodiesterase activity, and stabilize the released cAMP, which can then be analyzed directly in the microtiter plate.

Ref.: Chard, T. In: An Introduction to Radioimmunoassay and Related Techniques. 1990. 4th Ed., Elsevier, Amsterdam. NCCLS Evaluation Protocols, SC1, 1989, NCCLS, Villanova, PA, 19085. Tijssen, P. In: Practice and Theory of Enzyme Immunoassays. 1985. Elsevier, Amsterdam. Chabardes, D., et al. 1980. J. Clin. Invest. 65, 439. Grill, V., and Cerasi, E. 1974. J. Biol. Chem. 249, 41961. Szentivanyi, A. 1968. J. Allergy 42, 203. Haynes, R.C. 1958. J. Biol. Chem. 233, 1220.

R: 11-20/21-28-32-35-50-53; S: 26-3-16-27-28-29-36/37/39-45