w-Aga-IVA

Lyophilized. Potent selective high affinity blocker of P-type Ca²⁺ channels (K_d = 2 nM). Reported to abolish nicotine-evoked responses in cardiac vagal neurons (~100 nM). Biological activity: Fully inhibits the CA²⁺ current in a whole-cell patch-clamp assay at 50 nM using Purkinje neurons.. Purity: ≥99% by HPLC; single spot by TLC.

H-Lys-Lys-Lys-Cys⁴-Ile-Ala-Lys-Asp-Tyr-Gly-Arg-Cys¹²-Lys-Trp-Gly-Gly-Thr-Pro-Cys¹⁹-Cys²⁰-Arg-Gly-Arg-Gly-Cys²⁵-Ile-Cys²⁷-Ser-Ile-Met-Gly-Thr-Asn-Cys³⁴-Glu-Cys³⁶-Lys-Pro-Arg-Leu-Ile-Met-Glu-Gly-Leu-Gly-Leu-Ala-OH (disulfide bonds: 4 → 20; 12 → 25; 19 → 36; 27 → 34)

Ref.: Wang, J., et al. 2001. J. Neurophysiol. 85, 164. Nooney, J.M., and Lodge, D. 1996. Eur. J. Pharmacol. 306, 41. Mintz, I.M., et al. 1992. Nature 355, 827. Turner, T.J., et al. 1992. Science 258, 310.
R: 20/21/22; S: 36