Protein Kinase B Activity Assay Kit

Format: Immunoblot

Form: 40 Tests

Detection Method: Immunoblot

Species Reactivity: human, mouse, rat

Assay Time: 4 h

Sample Type: Cell lysates, tissue extracts

Kit Contents: Kinase Extraction Buffer, Akt Antibody, Protein A Agarose, GSK-3a Protein/ATP Mixture, Kinase Assay Buffer, GSK-3a, Phospho-Specific Antibody, and a user protocol.

Comments: Useful for the non-radioactive assay of Akt activity in cell lysate or tissue extract lysates. Akt is first enriched via immunoprecipitation with an anti-Akt antibody and then tested for its ability to phosphorylate GSK-3a, an Akt substrate. Phosphorylated GSK-3a is detected through immunoblotting with anti-GSK-3a phospho-specific antibody. Physiologically, GSK-3a is inactivated by Akt phosphorylation. Akt is activated by diverse stimuli such as growth factors and regulates many cellular processes such as apoptosis, metabolism, and proliferation through interactions with a variety of substrates.

Note: 1 kit = 40 assays.

Ref.: Nickolson, K.M., and Anderson, N.G. 2002. Cell Signal. 14, 381. van Weeren, P.C., et al. 1998. J. Biol. Chem. 273, 13150.