10-(4′-(N-diethylamino)butyl)-2-chlorophenoxazine, HCl White solid. PROTECT FROM LIGHT. HYGROSCOPIC. PACKAGED UNDER INERT GAS. A cell-permeable and selective inhibitor of the phosphorylation of Akt and its in vitro kinase activity (complete inhibition <5 µM) with minimal effect on PI 3-K, PDK1, or SGK1. Shown to suppress growth of Rh (rhabdomyosarcoma) cell lines (IC50 = 2-5 µM), inhibit IGF-I-stimulated nuclear translocation of Akt, and prevent phosphorylation of the downstream targets, mTOR, p70S6 kinase, and S6 ribosomal protein. Unlike Akti1/2 (Cat. No. 124018), the mode of inhibition is not PH domain-dependent. Purity: ≥95% by HPLC. CAS 925681-41-0. Ref.: Thimmaiah, K.N., et al. 2005. J. Biol. Chem. 280, 31924. |
