APN/CD13, aminopeptidase N

Liquid. In 200 mM NaCl, 50 mM Tris-HCl, pH 8.0. AVOID FREEZE/THAW CYCLES. Native aminopeptidase N purified from rat renal brush-border membranes and cleaved with papain to produce soluble enzyme. A single-chain, integral membrane protease that, functions with broad specificity to remove N-terminal amino acids from peptides. It has been reported that levels of aminopeptidase N are elevated in cancer subjects and correlate with tumor load, and thus, the enzyme is believed to play a role in angiogenesis. Specific activity: ≥100 units/mg protein. One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol L-Leu-pNA per min at 37°C, pH 7.2 Purity: ≥90% by SDS-PAGE. EC 3.4.11.2.

Ref.: Bhagwat, S.V., et al. 2003. Blood 101, 1818. van Hensbergen, Y., et al. 2002. Clin. Cancer Res. 8, 3747. Ishii, K., et al. 2001. Biol. Pharm. Bull. 24, 226.