APN/CD13, Aminopeptidase N Liquid. In 150 mM NaCl, 50 mM Tris-HCl, 1 mM CaCl2, pH 8.0. AVOID FREEZE/THAW CYCLES. Native aminopeptidase N purified from human serum. Aminopeptidase N is a single-chain, integral membrane protease with broad specificity that functions to remove N-terminal amino acids from peptides. It also acts as a receptor for coronavirus and is known to play a role in angiogenesis, HIV entry, and in degradation of extracellular matrix as a result of tumor invasion. Specific activity: ≥450 mU/mg protein. One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol L-leucine-p-nitroanilide) per min at 37°C, pH 7.2. Purity: ≥92% by SDS-PAGE. Prepared from human serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. EC 3.4.11.2. Ref.: Bhagwat, S.V., et al. 2003. Blood 101, 1818. Kawai, M., et al. 2001. Clin. Chem. 47, 223. Watanabe, Y., et al. 1998. Mol. Genet. Metab. 63, 289. Pulido-Cejudo, G., et al. 1997. Antiviral Res. 36, 167. Hansen, A.S., et al. 1993. Eur. J. Immunol. 23, 2358. Saiki, I., et al. 1993. Int. J. Cancer 54,137. Shipp, M.A. and Look, A.T. 1993. Blood 82, 1052. Yeager, C.L., et al. 1992. Nature 357, 420. |
