Liquid. In 150 mM NaCl, 50 mM Tris-HCL, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol, pH 7.5. AVOID FREEZE/THAW CYCLES. Full length, recombinant, human AMPK subunits (A1, B1, and G1), each fused at the C-terminus to a His•Tag^® sequence and co-expressed in S. frugiperda insect cells using a baculovirus expression system. AMPK is involved in sensing energy levels in the cell that result from changes in AMP levels. AMPK is an upstream regulator of mTOR activation. The active complex is useful in the study of kinase activity regulation and inhibitor screening. Specific activity: ≥2755 nmol/min/mg protein. Kinase activity is measured as the molar amount of phosphate incorporated into SAMS peptide substrate (HMRSAMSGLHLVKRR) at 30°C using a final concentration of 50 µM ATP. Purity: ≥95% by SDS-PAGE. M.W. = 68,000 (A1), 38,000 (B1), 40,000 (G1)

Ref.: Scott J.W., 2007. EMBO J 26, 806. Minokoshi Y et al. 2004. Nature 428, 569.