Solid. Lyophilized from sterile-filtered PBS containing 50 µg BSA/µg Angiogenin. Recombinant, human angiogenin expressed in E. coli. Exhibits angiogenic and ribonucleolytic activities. Enhances the ability of endothelial cells to digest extracellular matrix components and degrade basement membrane. Has been shown to stimulate capillary and umbilical vein endothelial cells to produce DAG and secrete prostacyclin by phospholipase activation. Biological activity: 1.0 µg protein produces an absorbance change at 260 nm of approximately 2.0 - 3.0 as measured by yeast tRNA ribonucleolytic activity. Biological activity: ≥1.0 µg protein produces an absorbance change at 260 nm of ~2.0-3.0 as measured based upon its ribonucleolytic activity toward Yeast tRNA.. Purity: ≥97% by SDS-PAGE. Contaminants: Endotoxins: ≤100 pg/µg Angiogenin.

Ref.: Leland, P.A., et al. 2002. Biochemistry 41, 1343. Hatzi, E., et al. 2000. Biochem. Biophys. Res. Commun. 267, 719. Hu, G., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 12096. Lee, F.S., and Vallee, B.L. 1989. Biochem. Biophys. Res. Commun. 161, 121. Kurachi, R., et al. 1985. Biochemistry 24, 5494.