Anthrax LF Protease Substrate III, Fluorogenic
Bacillus anthracis LF Protease Substrate III, Fluorogenic

Lyophilized. Supplied as a trifluoroacetate salt. PROTECT FROM LIGHT. HYGROSCOPIC. PACKAGED UNDER INERT GAS. An N-acetylated, C-7-amido-4-methylcoumarin (AMC) derivative of a 14-mer peptide substrate designed from the MEK-2 template that is useful for measuring Anthrax lethal factor (LF) metalloproteolytic activity with a detection limit of ~5-10 pM. Reported to be useful for high-throughput screening of LF inhibitors. Purity: ≥97% by HPLC. Excitation max.: ~360 nm, Emission max.: ~460 nm

Ac-Gly-Tyr-bAla-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Val-Leu-Arg-AMC

Ref.: Tonello, F., et. al. 2002. Nature 418, 386.