1,2-bis(o-Aminophenoxy)ethane-N,N,N′,N′-tetraacetic Acid, 4Na Off-white solid. PROTECT FROM LIGHT. HYGROSCOPIC. Ca2+ chelator exhibiting a 105-fold greater affinity for Ca2+ (Kd = 110 nM) than for Mg2+. After BAPTA complexes with Ca2+, the UV spectrum shifts from 254 to 279 nm at pH >6.0. Useful for spectrophotometric monitoring of extracellular Ca2+ levels, especially transient phenomena, because of its insensitivity to pH. Useful for the measurement and control of Ca2+ levels in eggs, chromaffin cells, muscle cells, retinal cells, and pancreatic cells. In conjunction with certain formazan dyes, BAPTA can be used in a colorimetric assay for Mg2+. Has neuroprotective effects in vitro against excitotoxic damage and spontaneous recurrent seizures, such as epilepsy. CAS 126824-24-6. Ref.: DeLorenzo, R.J., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 14482. Li, L., et al. 1995. J. Cell Physiol. 163, 105. Tymianski, M., et al. 1994. J. Neurophysiol. 72, 1973.
R: 36/37/38; S: 26-36 |
